We narrowed to 20,498 results for: ACE

1. 10TALE_Pmin_fLuc

   Plasmid

   #160663

   Purpose

   Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain

   Depositor

   Roman Jerala

   Article

   Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

   Insert

   10TALE_Pmin_fLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH41421

   Plasmid

   #50339

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV_Stargazin-mTurquoise2-iLID

   Plasmid

   #161001

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   Stargazin-mTurquoise2-iLID
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDestattB (pCM268)

   Plasmid

   #68313

   Purpose

   Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish

   Depositor

   Leonard Zon, Christian Mosimann

   Article

   Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

   Type

   Empty backbone

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJYScr

   Plasmid

   #132719

   Purpose

   Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance

   Depositor

   Jan Marienhagen

   Article

   Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24_NusA-Tus

   Plasmid

   #165959

   Purpose

   Expression of soluble E. coli Tus protein

   Depositor

   Keith Pardee

   Article

   Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

   Insert

   Tus
   

   Use

   Tags

   His-NusA-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. evoBE4-NG

   Plasmid

   #226852

   Purpose

   Expresses evoBE4max-NG base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   evoBE4max-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay-Rncp-iGluSnFR1

   Plasmid

   #107336

   Purpose

   Red fluorescent glutamate sensor with ncp topology. Membrane-displayed

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

   Insert

   Rncp-iGluSnFR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pREDusk-StrR-MCS

   Plasmid

   #188972

   Purpose

   For red light-repressed bacterial expression of gene of interest (StrR)

   Depositor

   Heikki Takala

   Article

   Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPP7CP-myc-T2A-tagRFP

    Plasmid

    #140262

    Purpose

    Expresses PP7CP in mammalian cells

    Depositor

    Hirohide Saito

    Article

    Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

    Insert

    Coat protein of bacteriphage PP7
    

    Use

    Synthetic Biology

    Tags

    myc-T2A-tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR-dCas9

    Plasmid

    #125687

    Purpose

    Gene knockdown for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spdCas9, sgRNA casette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDRF1-GW yAT1.03R122KR126K

    Plasmid

    #132782

    Purpose

    Non-binding version of the FRET biosensor for ATP measurements in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

    Insert

    yAT1.03R122KR126K ymTq2-tdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R122K, R126K

    Promoter

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET22b-6h-GST-TEVp

    Plasmid

    #172887

    Purpose

    Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Insert

    TEV protease
    

    Use

    Tags

    GST solubility tag and HHHHHH

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTPGI_dCas9_VP64

    Plasmid

    #49013

    Purpose

    encodes yeast-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    pTPGI (galactose+aTc inducible)

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. piggyBac-ancBE4-NG

    Plasmid

    #226865

    Purpose

    Expresses dox-inducible ancBE4max-NG for mammalian cells. piggyBac vector

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ancBE4max-NG
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Tet-On-TRE3G

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3_UnaG-Flag-Sec61B

    Plasmid

    #83413

    Purpose

    Expressed N-term tagged UnaG on Sec61B

    Depositor

    Hyun-Woo Rhee

    Article

    Park et al ACS Chem Biol. 2016 May 27.

    Insert

    UnaG-Sec61B (SEC61B Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSHDY-Prha-mVenus_rhaS

    Plasmid

    #137662

    Purpose

    Conjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.

    Depositor

    Ilka Axmann

    Article

    Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

    Insert

    Prha:mVenus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    rhaS (Arg218Leu)

    Promoter

    Prha

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19-T7-3WJdB-T

    Plasmid

    #87308

    Purpose

    Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Three-way junction dimeric Broccoli aptamer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBad-UGAcS

    Plasmid

    #172137

    Purpose

    bacteria expression plasmid for a UDP-GlcNAc sensor

    Depositor

    Huiwang Ai

    Article

    Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

    Insert

    UGAcS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H605Q- please see depositor comments

    Promoter

    araBAD

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCpf1

    Plasmid

    #122185

    Purpose

    Bacterial genome editing plasmid with kanamycin resistance marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge