We narrowed to 4,109 results for: Mcc;

1. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

   Plasmid

   #169160

   Purpose

   To express nsp14-nsp10 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tet-O-FUW-GLI1-P2A-mCherry

   Plasmid

   #203858

   Purpose

   Tet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   GLI1 (GLI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)

   Plasmid

   #159687

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)

   Depositor

   Dominic Esposito

   Article

   Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

   Insert

   His6-MBP-tev-Hs.RAF1(52-192) (RAF1 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. VARP GFP-pLXIN

   Plasmid

   #62950

   Purpose

   full length human VARP with C-terminal EGFP tag cloned into pLXIN

   Depositor

   Paul Luzio

   Article

   Hesketh et al Dev Cell. 2014 Jun 9;29(5):591-606. doi:

   Insert

   VARP (ANKRD27 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silent mutations of amino acids 422-428 to produc…

   Promoter

   LTR

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

   Plasmid

   #159700

   Purpose

   E. coli protein expression

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)
   

   Use

   Tags

   His6-MBP-tev-Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

   Plasmid

   #107582

   Purpose

   B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   GFP+
   

   Use

   Synthetic Biology; E. coli and bacillus shuttle v…

   Tags

   B. megaterium DSM319 xylA leader sequence (MTSSKI…

   Expression

   Bacterial

   Mutation

   F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

   Promoter

   fba promoter Bacillus megaterium DSM319

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET11a_3xFlag-nsp14 (SARS-CoV-2)

   Plasmid

   #169159

   Purpose

   To express SARS-CoV-2 nsp14 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.23 C3_7

   Plasmid

   #60293

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   SLC30A8 enhancer (SLC30A8 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. Tet-O-FUW-KLF4-P2A-mCherry

   Plasmid

   #203855

   Purpose

   Tet-inducible lentiviral plasmid expressing KLF4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tet-O-FUW-MAFB-P2A-mCherry

    Plasmid

    #203866

    Purpose

    Tet-inducible lentiviral plasmid expressing MAFB-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    MAFB (MAFB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-hCCND1-T286A-HA

    Plasmid

    #174155

    Purpose

    Lentiviral vector expressing HA-tagged human cyclin D1 with T286A mutation

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    A>G at base 856 (Thr>Ala at amino acid 286)

    Promoter

    EF1A

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-PGK-EGFP-ALDH1A3-Neo

    Plasmid

    #189744

    Purpose

    The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A3 (ALDH1A3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tet-O-FUW-ID2-P2A-mCherry

    Plasmid

    #203865

    Purpose

    Tet-inducible lentiviral plasmid expressing ID2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    ID2 (ID2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4-deltaH-AMBRA1-3xFLAG

    Plasmid

    #174158

    Purpose

    Vector for expression of 3xFLAG-tagged truncated AMBRA1 that cannot bind DDB1 (aa 1-34 deleted)

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 1-34

    Promoter

    CMV

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a-TALEN_HD (SPC right)

    Plasmid

    #126695

    Purpose

    right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

    Depositor

    Darrell Kotton

    Article

    Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

    Insert

    human SPC right
    

    Use

    TALEN

    Tags

    NLS, truncated N term and TAL, FokI

    Expression

    Mammalian

    Mutation

    R451P in SPC

    Promoter

    EF1

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a-TALEN_NN (SPC left)

    Plasmid

    #126694

    Purpose

    left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

    Depositor

    Darrell Kotton

    Article

    Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

    Insert

    human SPC left
    

    Use

    TALEN

    Tags

    0.5 repeat NN, TAL, FokI and NLS, truncated N ter…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP

    Plasmid

    #75123

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    3x FLAG-tag

    Expression

    Mutation

    bromodomain replaced with the first bromodomain o…

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

18. lentiCRISPRv2_AMBRA1#2

    Plasmid

    #174153

    Purpose

    Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA (AMBRA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKHH030_sgRB1#1

    Plasmid

    #174144

    Purpose

    Lentiviral vector expressing a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

    Plasmid

    #75126

    Purpose

    Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    KMT6 (EZH2 Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    SET domain replaced with the SET domain of EZH1

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only