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pEF5-H4Δ1-19-HaloTag-FRT

Plasmid

#247456

Purpose

Expresses wild-type H4(Δ1-19)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4Δ1-19 lacks the initial 19 residues at the N-te…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pIG-749_HA-GD2-28z_CAR_TFAP4

Plasmid

#207490

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-852_HA-GD2-28z_TFAP4-HA

Plasmid

#207502

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-858_CD19-BBz_CAR_TFAP4

Plasmid

#207503

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-BBz_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-Cas9-2A-BFP

Plasmid

#164662

Purpose

Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

Cas9
BFP

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

MSSV_LTR

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pIG-829_HA-GD2-28z_CAR_JUN_Retroviral

Plasmid

#207506

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

JUN, HA-GD2-28z_CAR (JUN Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

Plasmid

#207507

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-828_HA-GD2-28z_CAR_BATF_Retroviral

Plasmid

#207505

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF, HA-GD2-28z_CAR (BATF Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-BCL2

Plasmid

#163611

Purpose

Overexpression of human BCL2

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Insert

BCL2 (BCL2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEF5-H4(A5,8,12,16)-HaloTag-FRT

Plasmid

#247455

Purpose

Expresses wild-type H4(A5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4(A5,8,12,16) has four mutations in the N-termin…

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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PB-EF1-H4Δ1-19-HaloTag-IRES-Neo

Plasmid

#247451

Purpose

Expresses H4Δ1-19-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4Δ1-19 lacks the initial 19 residues at the N-te…

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF-1α-Venus-Nup107-FRT

Plasmid

#247344

Purpose

Expresses Venus-NUP107 under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

Nup107 (NUP107) (NUP107 Human)

Tags

Venus

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4(Q5,8,12,16)-HaloTag-FRT

Plasmid

#247454

Purpose

Expresses wild-type H4(Q5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4(Q5,8,12,16) has four mutations in the N-termin…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF5-H4(R5,8,12,16)-HaloTag-FRT

Plasmid

#247453

Purpose

Expresses wild-type H4(R5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4(R5,8,12,16) has four mutations in the N-termin…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGGG L2 TaStb15

Plasmid

#226630

Purpose

Wheat transformation vector used to express wheat Septoria tritici blotch (Stb) resistance gene 15

Depositor

Brande Wulff

Article

Hafeez et al Nat Plants. 2025 Mar;11(3):410-420. doi:

Insert

Triticum aestivum (wheat) Septoria tritici blotch 15 (TaStb15) disease resistance gene

Use

Synthetic Biology

Expression

Plant

Promoter

Native TaStb15

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

Plasmid

#207493

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-851_HA-GD2-28z_HA-TFAP4

Plasmid

#207501

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HDAC2-H141A

Plasmid

#115346

Purpose

Expresses mouse HDAC2 cDNA with H141A point mutation

Depositor

Javier Gonzalez-Maeso

Article

Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

Insert

Histone deacetylase 2 (Hdac2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

H141A

Promoter

CMV

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pIG-804_HA-GD2-28z_CAR_BATF-TFAP4

Plasmid

#207491

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF-TFAP4, HA-GD2-28z_CAR (BATF Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-704_CD19-28z_CAR_tNGFR

Plasmid

#207486

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, CD19-28z_CAR (NGFR Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge