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mKate2-P2A-APEX2-TAPP1-PH

Plasmid

#67662

Purpose

Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-TAPP1-PH

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

Plasmid

#83919

Purpose

Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

DsRed-Express2
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmExRai-AKAR2

Plasmid

#161755

Purpose

Plasma membrane-targeted ExRai-AKAR2.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

Lyn-ExRai-AKAR2

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2p2A--3mnx1:mTagBFP-NS-MCS

Plasmid

#78250

Purpose

Can be used to subclone in gene of interest into blue motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-mTagBFP-NS-MCS

Use

Zebrafish motor neuron expression

Promoter

-3mnx1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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bact2-mKate2-P2A-TurboID-dVHH[GFP]

Plasmid

#163857

Purpose

Zebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBH

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID-VHH[EGFP]

Use

Zebrafish expression

Tags

mKate2-P2A-TurboID

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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GANES-DEVD-BNLS

Plasmid

#50842

Purpose

Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A and ddGFP B

Tags

NES sequence LALKLAGLDIGS placed after ddGFP A an…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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GANLS-DEVD-BNES

Plasmid

#50835

Purpose

Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A and ddRFP B

Tags

NES sequence LALKLAGLDIGS and triplicated NLS seq…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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single polypeptide FPX biosensor for caspase-8

Plasmid

#60884

Purpose

FPX biosensor for caspase-8

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for caspase-8

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
CAG-FR-GECO1a

Plasmid

#163681

Purpose

Far-red fluorescent calcium indicator FR-GECO1a

Depositor

Robert Campbell

Article

Dalangin et al Nat Commun. 2025 Apr 7;16(1):3318. doi:

Insert

FR-GECO1a

Expression

Mammalian

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-C1V1-eYFP

Plasmid

#135633

Purpose

AAV vector to drive the expression of C1V1-eYFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, AAV5, and AAV9

Insert

C1V1-eYFP

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Rab8-T22N(Flag) in pcDNA3.1neo

Plasmid

#46784

Purpose

expression of T22N Rab8a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB8A T22N (RAB8A Human)

Tags

FLAG

Expression

Mammalian

Mutation

T22N dominant negative

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-S5E2-C1V1-eYFP

Plasmid

#135633

Purpose

AAV vector to drive the expression of C1V1-eYFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, AAV5, and AAV9

Insert

C1V1-eYFP

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K11

Plasmid

#121152

Purpose

Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

All Ks apart from K11 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-ML1N*2

Plasmid

#67665

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1N*2

Expression

Mammalian

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD1 (full-length, aa 1-481)

Plasmid

#61405

Purpose

Expresses human OTUD1 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD2 (full-length, aa 1-348)

Plasmid

#61409

Purpose

Expresses human OTUD2 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-EB3mCherry_cmv-LifeActGFP

Plasmid

#133927

Purpose

Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EB3-mCherry and Lifeact-GFP (MAPRE3 Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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Ratiometric FPX sensor for ERK kinase activity

Plasmid

#60974

Purpose

For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A-WW domain-ERK substrate-ddFP B

Tags

ERK substrate is before ddFP copy B, WW domain is…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
Lenti-TetON-Sox2

Plasmid

#110280

Purpose

Doxycycline-inducible overexpression of Sox2

Depositor

Trudy Oliver

Article

Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

Insert

SRY (sex determining region Y)-box 2 (Sox2 Mouse)

Use

Lentiviral

Tags

No fusion genes

Expression

Mammalian

Mutation

Wild type

Promoter

TRE

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pDRM209 LTP (Luciferase tdTomato Puro)

Plasmid

#174723

Purpose

Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

Firefly Luciferase
tdTomato
pac

Use

Lentiviral and Luciferase

Tags

E2A linker and T2A linker

Expression

Mammalian

Promoter

MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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