We narrowed to 22,515 results for: tes

1. pDEST26 Ago2 4A

   Plasmid

   #59907

   Purpose

   Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   6xHis (N-termi tag)

   Expression

   Mammalian

   Mutation

   mutated four serines to alanines

   Promoter

   CMV

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tet-off Full length Tau (0N4R, P301L/V337M)

   Plasmid

   #188573

   Purpose

   Dox-repressible expression of the 0N4R Tau isoform

   Depositor

   Franz-Ulrich Hartl

   Article

   Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

   Insert

   0N4R Tau (MAPT Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   P301L and V337M

   Promoter

   pTight TRE

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB1alpha2_KanR_BastaR_Rb7_GFP

   Plasmid

   #186427

   Purpose

   optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Inserts

   KanR
   BastaR
   Rb7
   eGFP
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   CaMV 35S and Pnos

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS2 (CK2alpha', CK2beta)

   Plasmid

   #27093

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha' (CSNK2A2 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA and Myc

   Expression

   Mammalian

   Promoter

   bidirectional tet-responsive promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGV13 (CK2alpha K68M, CK2beta)

   Plasmid

   #27094

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha K68M (CSNK2A1 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA on CK2alpha and Myc on CK2beta

   Expression

   Mammalian

   Mutation

   K68M on CK2alpha

   Promoter

   bidirectional tet-responsive promoter and bidirec…

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. YFP(159-238)-gamma-2 in pcDNAI/Amp

   Plasmid

   #54472

   Purpose

   A carboxyl-terminal YFP fragment was fused to Ggamma-2. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(159-238)-gamma -2 (GNG2 Human, Aequorea victoria)
   

   Tags

   YFP(159-238) was fused to the amino terminus of G…

   Expression

   Mammalian

   Mutation

   Ggamma-2 was amplified by PCR, which added a BamH…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGV7 (CK2alpha' K69M, CK2beta)

   Plasmid

   #27095

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha' K69M (CSNK2A2 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA tag on CK2alpha' and Myc on CK2beta

   Expression

   Mammalian

   Mutation

   K69M on CK2alpha'

   Promoter

   bidirectional tet-responsive promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. YFP(159-238)-gamma-1 in pcDNAI/Amp

   Plasmid

   #54471

   Purpose

   A carboxyl-terminal YFP fragment was fused to Ggamma-1. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(159-238)-gamma-1 (GNGT1 Bovine, Aequorea victoria)
   

   Tags

   YFP(159-238) was fused to the amino terminus of G…

   Expression

   Mammalian

   Mutation

   Ggamma-1 was amplified by PCR, which added a Bam…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP(159-238)-gamma-7 in pcDNAI/Amp

   Plasmid

   #54473

   Purpose

   A carboxyl-terminal YFP fragment was fused to Ggamma-7. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(159-238)-gamma-7 (GNG7 Human, Aequorea victoria)
   

   Tags

   YFP(159-238) was fused to the amino terminus of G…

   Expression

   Mammalian

   Mutation

   Ggamma-7 was amplified by PCR, which added a BamH…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/hFGFR3-K650M-V5

    Plasmid

    #214909

    Purpose

    expression of the K650M mutant variant of human FGFR3, which is associated with Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans (SADDAN)

    Depositor

    Pavel Krejčí

    Article

    Kunova Bosakova et al Proc Natl Acad Sci U S A. 2019 Feb 19. p

    Insert

    FGFR3 (FGFR3 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    K650M substitution

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196084

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196078

    Purpose

    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid

    #54468

    Purpose

    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
    

    Tags

    YFP(1-158) was fused to the amino terminus of Gbe…

    Expression

    Mammalian

    Mutation

    GBeta-1 was amplified via PCR, which removed an i…

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196082

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196076

    Purpose

    (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196080

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. YFP(1-158)-beta-2 in pcDNAI/Amp

    Plasmid

    #54469

    Purpose

    An amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP(1-158)/beta-2 (GNB2 Human, Aequorea victoria)
    

    Tags

    YFP(1-158) was fused to the amino terminus of Gbe…

    Expression

    Mammalian

    Mutation

    YFP (1-158) includes a substitution of Met for Gl…

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196074

    Purpose

    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. YFP(1-158)-beta-5 in pcDNAI/Amp

    Plasmid

    #54470

    Purpose

    An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP(1-158)/beta-5 (GNB5 Human, Aequorea victoria)
    

    Tags

    YFP(1-158) was fused to the amino terminus of Gbe…

    Expression

    Mammalian

    Mutation

    YFP (1-158) includes a substitution of Met for Gl…

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. DLX2-hygro

    Plasmid

    #97330

    Purpose

    Generating lentiviruses to express Dlx2 and hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    DLX2 (Dlx2 Mouse)
    

    Use

    Lentiviral

    Promoter

    TetO

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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