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p5E-EF1a (JDW 1164)

Plasmid

#224473

Purpose

Gateway compatible 5' entry clone containing the human EF1a promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF1a promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

Plasmid

#218862

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8m

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

A25G F286Y

Promoter

hsp70

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-H2A_mCherry_SV40pA (JDW 967)

Plasmid

#224528

Purpose

A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2A-mCherry-SV40pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-C7

Plasmid

#194591

Purpose

Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

C7 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

Plasmid

#218867

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8f

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST47-CYFIP1.GFP

Plasmid

#109139

Purpose

GFP-tagged CYFIP1 expression construct

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP1 (CYFIP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X

Plasmid

#192148

Purpose

T cell CRISPR AAV vector

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Type

Empty backbone

Use

AAV

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor (all-in-one) ORF2

Plasmid

#200388

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pB-2x-lns-DEST (JDW 1206)

Plasmid

#229818

Purpose

A PiggyBac destination vector compatible with gateway with 2 core cHS4 insulators by each ITR site

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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CYFIP1.mCherry

Plasmid

#122051

Purpose

Expresses human CYFIP1 in mammalian cells.

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP1 (CYFIP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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