Addgene

We narrowed to 2,644 results for: GUS

Showing: 2281 - 2300 of 2644 results

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ER-RspA-CFAST

Plasmid

#233607

Purpose

Expression of RspA-CFAST on the ER membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-CFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPB9v2

Plasmid

#210029

Purpose

Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPB9P2

Plasmid

#210030

Purpose

Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

prime editor 2
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPB1163

Plasmid

#210032

Purpose

Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas11-P2A-Cas6-T2A-Cas3
rtta-T2A-blast

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPB758

Plasmid

#210033

Purpose

Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas7-P2A-Cas5-T2A-Cas8
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-FXR1-C (379-621)

Plasmid

#225451

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (FXR1 fragment (379-621))

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

C terminal fragment (379-621)

Promoter

CMV

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-N1-KH12mut (T236D H237D K299D N300D)

Plasmid

#225454

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (T236D H237D K299D N300D)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

T236D H237D K299D N300D

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL FBM*

Plasmid

#195220

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. With H477/R479/S481>AAA mutation.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Use

Tags

Expression

Mammalian

Mutation

H477/R479/S481>AAA

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL S481A

Plasmid

#195223

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphorylation-deficient mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Use

Tags

Expression

Mammalian

Mutation

S481A

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2 DomI + DomII pcDNA3

Plasmid

#206094

Purpose

expression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Bn CAAX pcDNA3

Plasmid

#206088

Purpose

Cav2.2 N-terminus (amino acids 1-95) with a C-terminal tag of the final 10 aa of H.Ras containing CAAX motif

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pCAGGS

Plasmid

#206101

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 W391A pRK5

Plasmid

#206084

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 DomI pMT2

Plasmid

#206127

Purpose

expression of the N-terminus, Domain I and the I-II loop (amino acids 1-483) of rabbit Cav2.2 calcium channel

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ad MLP/TPL/SV40

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA W391A pcDNA3

Plasmid

#206071

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1b in pT3TS-Dest

Plasmid

#194295

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

In vitro transcription of mrna

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S E63C

Plasmid

#190009

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, E63C

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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mEos2-dVHH[GFP] in pCSDEST

Plasmid

#162870

Purpose

Super-resolution of GFP in culture

Depositor

Rob Parton

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

mEos2-dVHH[EGFP]

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-Clover

Plasmid

#126572

Purpose

Multisite gateway vector for 3' tagging with Clover. Parton lab clone DJZ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Clover

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCu-His6-Atg13[571–700]-Ss(424)

Plasmid

#166852

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

pCu

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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