We narrowed to 170,873 results for: addgene
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Plasmid#58854PurposeConstitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)DepositorInserttTA transcription factor fused to enhanced blue fluorescent protein
TagsBFPExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
mTB3-BC2 nanobody
Plasmid#210208PurposeType 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.DepositorInsertBC2 Nanobody
ExpressionBacterialAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
7MEQ
Plasmid#176412PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertTMPRSS2āāātransmembrane serine protease 2 (TMPRSS2 Human)
UseBaculovirus expresssionTags8 x His Tag and Honeybee melittin signalMutationetmprss2_S251D/R252D/Q253D/S254D/R255KPromoterPolyhedrinAvailable SinceOct. 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
VV073: 1xCox8 - GCaMP5G in fubi
Plasmid#58509PurposeExpresses the calcium sensor GCaMP5G in the mitochondriaDepositorInsert1xCox8 - GCaMP5G
UseLentiviralExpressionMammalianPromotera-CamKIIAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
PNCA-GFP
Plasmid#67789PurposeUse together with pCMV-intron and VSV-G to package MMLV reporter virusDepositorInsertMMLV-LTR-GFP
Available SinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV/EF1a-DIO-SCLM
Plasmid#216759PurposeAAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoterDepositorInsertSuperClomeleon
UseAAVExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moietā¦PromoterEF1a (human elongation factor 1 alpha)Available SinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNM3689
Plasmid#154111PurposeminiMos single component RMCE landing site vectorDepositorInsertloxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
UseMinimosPromoternot applicableAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBI-MCS-EYFP
Plasmid#58855PurposeThe tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFPDepositorInsertenhanced yellow fluorescent protein
ExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::ZeoR-bGHpA
Plasmid#62442PurposeMXS_chaining vector with PGK::ZeoR-bGHpADepositorInsertresistance cassette against Zeocin with PGK Promoter
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
LES2A-CRE
Plasmid#167933PurposeLentiviral CRE expression tracked by mScarlet-I fluorescence marker.DepositorInsertmScarlet-P2A-CRE
UseCre/Lox and LentiviralExpressionMammalianPromoterEF1aAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pNH-NanoLuc
Plasmid#173075PurposeExpression vector for E. coli producing target protein with N-terminal Nanoluciferase fusionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8266 LentiCRISPR v2 hygro sgNT-1
Plasmid#193977PurposeExpression of spCas9 and non-targeting control sgRNADepositorInsertspCas9
UseLentiviralAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCP-EGFP-PiggyBac
Plasmid#190003PurposeExpression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.DepositorInsertMCP-EGFP
Available SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
LacZ_pHIVEGFP
Plasmid#191251PurposeExpresses LacZ in mammalian cells (used as a control)DepositorInsertLacZ
TagsEGFPExpressionMammalianPromoterEF1alphaAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATG16L1 sgRNA
Plasmid#207563PurposepX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locusDepositorInsertGCAGCAAGTGACATGTCGTC
ExpressionMammalianPromoterCMV and U6Available SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
HTATIP (2OU2)
Plasmid#33338PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP (KAT5 Human)
TagsHexahistidine tag with Thrombin cleavageExpressionBacterialPromoterT7Available SinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX459_gRNA-ROSA26_hspCas9
Plasmid#193310PurposeCas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)DepositorInsertCas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
UseCRISPRExpressionMammalianMutationnot applicableAvailable SinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-MCS-IRES-mRFP
Plasmid#36972DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-dCasRx-ccdB
Plasmid#221003PurposeDestination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only