We narrowed to 2,328 results for: dap

1. pDEST17-cDAXX-0SB

   Plasmid

   #169730

   Purpose

   Bacterial expression of N-terminally 6His tagged cDAXX-0SB

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   cDAXX-0SB (DAXX Human)
   

   Use

   Tags

   6 His

   Expression

   Bacterial

   Mutation

   Comprises residues 495-741 and has the major SB m…

   Promoter

   T7

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-VRER-P2A-GFP-PGK-Puro

   Plasmid

   #110862

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-HF1-P2A-GFP-PGK-Puro

   Plasmid

   #110863

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-CMV-6xHis-SUMO1

   Plasmid

   #164939

   Purpose

   Expresses 6xHis tagged human Small Ubiquitin Like Modifier 1 (SUMO1) wild type cDNA

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Small Ubiquitin Like Modifier 1 (SUMO1 Human)
   

   Use

   Lentiviral

   Tags

   6XHIS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-xF2X-P2A-Puro

   Plasmid

   #110873

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS-2X(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAPK3 gRNA (BRDN0001147876)

   Plasmid

   #75967

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAPK3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAPK3 (MAPK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAPK3 gRNA (BRDN0001147667)

   Plasmid

   #75968

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAPK3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAPK3 (MAPK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-myc-AP1S3

   Plasmid

   #58292

   Purpose

   mammalian expression of AP1S3

   Depositor

   Francesca Capon

   Article

   Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

   Insert

   AP1S3 (AP1S3 Human)
   

   Use

   Tags

   6xHis and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

9. MAPK3 gRNA (BRDN0001149014)

   Plasmid

   #75966

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAPK3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAPK3 (MAPK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF3372

    Plasmid

    #144848

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    DAXX (DAXX Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF1228

    Plasmid

    #142761

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    DAXX (DAXX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pInducer-myc-ERK1-R84S/S170D

    Plasmid

    #213593

    Purpose

    Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D cDNA in mammilian cells

    Depositor

    Channing Der

    Article

    Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    R84S, S170D

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer-myc-ERK1-R84S

    Plasmid

    #213591

    Purpose

    Doxycycline inducible expression of myc-tagged ERK1-R84S cDNA in mammilian cells

    Depositor

    Channing Der

    Article

    Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    R84S

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRIPZ-bioGEF-SUMO1nc-PURO

    Plasmid

    #208042

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO1nc (SUMO1 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Expression

    Mutation

    Q94P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-myc-AP1S3 F4C

    Plasmid

    #58293

    Purpose

    mammalian expression of AP1S3 F4C mutant

    Depositor

    Francesca Capon

    Article

    Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

    Insert

    AP1S3 (AP1S3 Human)
    

    Use

    Tags

    6xHis and myc

    Expression

    Mammalian

    Mutation

    F4C: c.11T>G, p.Phe4Cys

    Promoter

    CMV

    Available since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-myc-AP1S3 R33W

    Plasmid

    #58294

    Purpose

    mammalian expression of AP1S3 R33W mutant

    Depositor

    Francesca Capon

    Article

    Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

    Insert

    AP1S3 (AP1S3 Human)
    

    Use

    Tags

    6xHis and myc

    Expression

    Mammalian

    Mutation

    R33W: c.97C>T,p.Arg33Trp

    Promoter

    CMV

    Available since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pZE21/UBP1/ClpS_V65I

    Plasmid

    #98567

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains the V65I mutation that increases discrim…

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pInducer-myc-ERK1-S170D

    Plasmid

    #213592

    Purpose

    Doxycycline inducible expression of myc-tagged ERK1-S170D cDNA in mammilian cells

    Depositor

    Channing Der

    Article

    Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    S170D

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pInducer-myc-ERK1-R84S/D338N

    Plasmid

    #213594

    Purpose

    Doxycycline inducible expression of myc-tagged ERK1-R84S/D338N cDNA in mammilian cells

    Depositor

    Channing Der

    Article

    Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    R84S, D338N

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid

    #208035

    Purpose

    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    SUMO1nc (SUMO1 Human)
    

    Use

    Lentiviral

    Tags

    Myc, C-terminal Split-TurboID

    Expression

    Mutation

    Q94P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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