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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,282 results for: golden gate

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Showing: 2301 - 2320 of 5282 results

  3. pIF1079

    Plasmid
    #237445
    Purpose
    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.
    Depositor
    IIya Finkelstein
    Publication
    Buffington et al Nat Biotechnol. 2025 Oct 23. doi: 10.103
    Inserts
    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CAG, H1, and U6
    Available since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    237445-plasmid-map-sequence-id-476973

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  4. Komagataeibacter Tool Kit (KTK)

    Plasmid kit
    #1000000216
    Purpose
    A collection of standardized parts used for hierarchical assembly of single and multi-gene constructs for Komagataeibacter species.
    Depositor
    Tom Ellis
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only

  10. Level 1 P1 OsActinP:Hygint:NosT

    Plasmid
    #165423
    Purpose
    GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Publication
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
    Use
    Synthetic Biology; L1 p1(r) golden gate (moclo) h…
    Promoter
    Os Actin promoter :: Hygromycin resistance gene (…
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    165423-plasmid-map-sequence-id-331267

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  11. Start-Stop Assembly Toolkit

    Plasmid kit
    #1000000152
    Purpose
    Modular, scarless DNA assembly method suitable for combinatorial assembly and ideal for metabolic engineering.
    Depositor
    John Heap
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 2301 - 2320 of 5282 results