We narrowed to 9,615 results for: ef1a

1. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

   Plasmid

   #175573

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167937

   Purpose

   Inducible CRISPRa expression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   dxCas9(3.7)-VPR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   tetON

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR

   Plasmid

   #213481

   Purpose

   AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   GFP-KASH
   U6-gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

   Plasmid

   #183411

   Purpose

   piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   Tet-on 3G
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro

   Plasmid

   #141372

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp6 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

   Plasmid

   #175574

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40

   Plasmid

   #124603

   Purpose

   Cre-dependent soma-localized ChrimsonR for optogenetic stimulation

   Depositor

   Christopher Harvey

   Article

   Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

   Has Service

   AAV9

   Insert

   Soma-localized ChrimsonR
   

   Use

   AAV

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

   Plasmid

   #141378

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

   Plasmid

   #141373

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

    Plasmid

    #141374

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

    Plasmid

    #141383

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid

    #220612

    Purpose

    Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-ArgiNLS-AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

    Plasmid

    #141370

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro

    Plasmid

    #141367

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro

    Plasmid

    #141390

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf8 (ORF8 SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro

    Plasmid

    #141379

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro

    Plasmid

    #141368

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp2 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro

    Plasmid

    #141380

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pLVX-EF1alpha-SARS-CoV-2-nsp10-2xStrep-IRES-Puro

    Plasmid

    #141376

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pLVX-EF1alpha-SARS-CoV-2-orf7a-2xStrep-IRES-Puro

    Plasmid

    #141388

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf7a (ORF7a SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge