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C078

Plasmid

#174347

Purpose

Template for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

ePAB

Use

In vitro transcription template

Tags

FLAG

Expression

Mutation

13 silent mutations: G243A, A273G, G339A, C345G, …

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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C233

Plasmid

#174349

Purpose

Template for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (M161A) (pabpc1.S Frog)

Use

In vitro transcription template

Tags

FLAG

Expression

Mutation

M161A

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

Plasmid

#167935

Purpose

Inducible CRISPRi expression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

KRAB-dCas9-DHFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

tetON

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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35S-GWY-GAL4BD/pAM

Plasmid

#201231

Purpose

Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GWY-GAL4BD

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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35S-GAL4BD-GWY/pAM

Plasmid

#201233

Purpose

Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GAL4BD-GWY

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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HLTV-hTRIM21

Plasmid

#104973

Purpose

Purification of His-Lipoyl-hTRIM21 protein from bacteria

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (TRIM21 Human)

Use

Tags

His-Lipoyl

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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p323-L10A-PATagRFP

Plasmid

#74172

Purpose

Fluorescent reporter for single ribosome tracking

Depositor

Robert Singer

Article

Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

Insert

ribosomal protein L10A (Rpl10a Rat)

Use

Lentiviral

Tags

PATagRFP

Expression

Mutation

Promoter

UbC

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_AGIA-AirID

Plasmid

#182210

Purpose

Express AirID in mammalian cells.

Depositor

Tatsuya Sawasaki, Akira Nozawa, Shogo Nakano, Sohei Ito

Article

Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

Insert

AGIA-AirID

Use

Tags

AGIA-tag

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET-11b_PcrA_M6_H93A

Plasmid

#194156

Purpose

Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

Depositor

Taekjip Ha

Article

Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

Insert

Engineered helicase PcrA_M6

Use

Tags

6x His tag and FLAG tag

Expression

Bacterial

Mutation

H93A, C96A, N187C, C247A, L384V, L409C

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSMPP-mCherry-hTRIM21

Plasmid

#104972

Purpose

Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (TRIM21 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

SFFV

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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