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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,523 results for: golden gate

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Showing: 2361 - 2380 of 5523 results

  3. pBFC0993

    Plasmid
    #231993
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer
    Depositor
    Brady Cress
    Publication
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and pTet
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231993-plasmid-map-sequence-id-467616

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  4. pBG-ttLbUV2

    Plasmid
    #231386
    Purpose
    LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.
    Depositor
    Qi-Jun Chen
    Publication
    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:
    Insert
    ttLbCas12a Ultra V2
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D156R and E795L
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    231386-plasmid-map-sequence-id-461803

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  11. pBLU-VIPR

    Plasmid
    #220498
    Purpose
    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Publication
    Pulgarin et al Nucleic Acids Res. 2025 Mar 20;53(6):gka
    Type
    Empty backbone
    Use
    CRISPR; Transposon
    Expression
    Mammalian
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    220498-plasmid-map-sequence-id-442816

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  13. EXPRESSYALI

    Plasmid kit
    #1000000245
    Purpose
    Assembly of expression constructs for Yarrowia lipolytica.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 2361 - 2380 of 5523 results