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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,151 results for: nes

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Showing: 2361 - 2380 of 17151 results

  1. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84240
    Purpose
    Expresses GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    84240-plasmid-map-sequence-id-160812

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  3. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    68708-plasmid-map-sequence-id-123989

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  4. SPACE (pRZ1813)

    Plasmid
    #140242
    Purpose
    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPACE
    Expression
    Mammalian
    Mutation
    V82G in TadA*, D10A in Cas9, R187W in pmCDA1
    Promoter
    CMV
    Available since
    June 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    140242-plasmid-map-sequence-id-275730

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  7. px459 VQR

    Plasmid
    #101715
    Purpose
    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VQR (D1135V, R1335Q and T1337R)
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    101715-plasmid-map-sequence-id-196971

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  9. Flag-Jun4A-Myc

    Plasmid
    #47444
    Purpose
    mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)
    Depositor
    Axel Behrens
    Publication
    Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Jun 4A mutant (Jun Mouse)
    Tags
    Flag and myc
    Expression
    Mammalian
    Mutation
    four N-terminal residues phosphorylated by JNK, s…
    Promoter
    CMV
    Available since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    47444-plasmid-map-sequence-id-244398

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  15. pCK668

    Plasmid
    #192637
    Purpose
    Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Publication
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Sp.pCas9-dCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Mutation
    SoxS has R93A and S101A mutations and dCas9-NG ha…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192637-plasmid-map-sequence-id-381515

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  16. pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

    Plasmid
    #123342
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cerulean3-Cerulean3-FLARE-EKAR-EV
    Tags
    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123342-plasmid-map-sequence-id-307086

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  17. pcDNA3-mCherry-mCherry-FLARE-EKAR-EV

    Plasmid
    #123341
    Purpose
    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-mCherry-FLARE-EKAR-EV
    Tags
    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123341-plasmid-map-sequence-id-307074

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  18. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

    Plasmid
    #123330
    Purpose
    Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus-cpVenus-FLARE-AKAR(T/A)
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    123330-plasmid-map-sequence-id-306911

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Showing: 2361 - 2380 of 17151 results