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Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75243

Purpose

CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75242

Purpose

CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)

Plasmid

#206461

Purpose

Oskar 139-240 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-240 (osk Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 388-606 (OSK domain) (HK75)

Plasmid

#206463

Purpose

Oskar 388-606 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 388-606 (osk Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 139-240 MUT (A162E/L228E) (HK202)

Plasmid

#206464

Purpose

Oskar 139-240 mut expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-240 mut (osk Fly)

Tags

PH mcherry

Expression

Insect

Mutation

A162E L228E)

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBR BsmAIM

Plasmid

#206206

Purpose

Constitutive expression of BsmAIM methyltransferase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis T4 DNA Ligase

Plasmid

#206207

Purpose

Inducible expression of T4 DNA ligase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

T4 DNA ligase

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-MMLV RT

Plasmid

#178797

Purpose

Inducible expression of MMLV RT in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

MMLV RT

Expression

Bacterial

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Expression

Bacterial

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Pol

Plasmid

#178782

Purpose

Inducible expression of Taq DNA polymerase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Polymerase

Expression

Bacterial

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK503

Plasmid

#110616

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBR BsmAIM Kan + p15A BsaIR Amp

Plasmid

#206208

Purpose

Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM and BsaIR

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBTK619

Plasmid

#110619

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK527a

Plasmid

#110606

Purpose

BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK107

Plasmid

#110575

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP25 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK620

Plasmid

#110620

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Jan. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK621

Plasmid

#110621

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK229

Plasmid

#110591

Purpose

BTK Type 3 coding sequence for golden gate assembly and homologous recombination

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Kanamycin resistance

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK527

Plasmid

#110605

Purpose

BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK510

Plasmid

#110611

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK599

Plasmid

#110600

Purpose

BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP dropout

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK509

Plasmid

#110610

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK121

Plasmid

#110581

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

PA3 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK102

Plasmid

#110574

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

T7 promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK124

Plasmid

#110583

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP25 promoter with lac operator and RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK001

Plasmid

#110573

Purpose

Broad-host-range toolkit (BTK) entry vector, with superfolder GFP

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK300

Plasmid

#110592

Purpose

BTK Type 4 terminator for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

rpoC

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK130

Plasmid

#110584

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

T7 promoter with lac operator and RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK206

Plasmid

#110588

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK599s

Plasmid

#110601

Purpose

BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP dropout

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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