Addgene

We narrowed to 503 results for: PA-GFP

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pCRII-TOPO CMV-cGFP-mMEN b_3' Sense

Plasmid

#46840

Purpose

Express cGFP transcript ending in the wildtype MEN beta triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP

Plasmid

#234881

Purpose

L1HS-silencing plasmid (CRISPRi gRNA2)

Depositor

Johan Jakobsson

Article

Adami et al bioRxiv 2025.01.17.633315

Insert

LacZ gRNA

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-NNQ-HA-GFP

Plasmid

#121164

Purpose

Expresses mmPtch1-B NNQ variant

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, N…

Promoter

CMV

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP

Plasmid

#120909

Purpose

Expresses Ptch1-B (I766FV111FL114F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP

Plasmid

#120908

Purpose

Expresses Ptch1-B (V111FL114FW115A)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP

Plasmid

#120907

Purpose

Expresses Ptch1-B (I766FV111F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-MCU-EGFP

Plasmid

#181866

Purpose

Expresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

Insert

mitochondrial calcium uniporter (Mcu Mouse)

Use

AAV and Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A+sgGFP

Plasmid

#213165

Purpose

Vector with sgGFP for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgGFP

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa and U6 and PGK

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense

Plasmid

#46835

Purpose

Express cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense

Plasmid

#46836

Purpose

Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

Plasmid

#46839

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

Plasmid

#46838

Purpose

Express cGFP transcript ending in the minimal functional MALAT1 triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense

Plasmid

#46837

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

Plasmid

#174893

Purpose

amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Insert

mCherry and EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

Plasmid

#234882

Purpose

L1HS-silencing plasmid (CRISPRi gRNA1)

Depositor

Johan Jakobsson

Article

Adami et al bioRxiv 2025.01.17.633315

Insert

L1HS-silencing plasmid (CRISPRi gRNA1)

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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ARR3tk-eGFP/SV40-mCherry

Plasmid

#132360

Purpose

To measure transcriptional activity of Androgen Receptor

Depositor

Charles Sawyers

Article

Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

Insert

AR responsive elements (AR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSAD-5PSD95-EGFP-SynPhRFP

Plasmid

#217979

Purpose

G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

Depositor

Edward Callaway

Article

Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

Inserts

5PSD95-EGFP (Dlg4 Rat)
SynPhRFP (Syp Mouse)

Use

G-deleted rabies

Tags

EGFP and RFP

Expression

Mutation

Promoter

None

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A-inactive+sgGFP

Plasmid

#213169

Purpose

Vector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgGFP

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa and U6

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pALS2-sfGFP WT

Plasmid

#197575

Purpose

Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
M. alvus Pyl-tRNA (6)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

araC and lpp

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Mb-sfGFP WT

Plasmid

#197569

Purpose

Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
Pyl-tRNA (4 copies)

Use

Tags

V5-His6

Expression

Mammalian

Mutation

Promoter

CMV and U6/H1

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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