We narrowed to 1,231 results for: POLI

1. GFP-polyQ23-NLS

   Plasmid

   #231891

   Purpose

   Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q23-NLS (HTT Human)
   

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-MS2V5-eIF4G delN83

   Plasmid

   #65812

   Purpose

   expression of eIF4G-MS2 fusion.

   Depositor

   Niels Gehring

   Article

   Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

   Insert

   eIF4G (EIF4G1 Human)
   

   Tags

   MS2 stem loops and V5

   Expression

   Mammalian

   Mutation

   delN83 (functionally behaves as the WT protein)

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-MS2V5-eRF3a F76a

   Plasmid

   #65811

   Purpose

   expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1

   Depositor

   Niels Gehring

   Article

   Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

   Insert

   eRF3a (GSPT1 Human)
   

   Tags

   MS2 stem loops and V5

   Expression

   Mammalian

   Mutation

   F76a

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG32-3HA-3FLAG

   Plasmid

   #154081

   Purpose

   For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

   Depositor

   Cornelia Kilchert

   Article

   Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

   Insert

   3xHA-3xFLAG-hygR
   

   Use

   Pcr-based tagging in yeast

   Tags

   3xHA-3xFLAG

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1A-PARP14-1535-1601-Hygro

   Plasmid

   #238100

   Purpose

   Expression of structurally predicted WWE domain of PARP14 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   PARP14 (PARP14 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1535-1601 only

   Promoter

   EF1alpha

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Hygro-EF1A-TRIP12(749-836)-EGFP

   Plasmid

   #196229

   Purpose

   EGFP fused to the C-terminus of a WWE domain within TRIP12 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   C-terminal EGFP tagged TRIP12(749- 836)
   

   Use

   Lentiviral

   Tags

   EGFP and Myc tag

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Hygro-EF1A-DTX2(8-97)-EGFP

   Plasmid

   #196230

   Purpose

   EGFP fused to the C-terminus of first WWE domain within Deltex2 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   C-terminal EGFP tagged DTX2(8-97)
   

   Use

   Lentiviral

   Tags

   EGFP and Myc tag

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-Hygro-EF1A-DTX2(8-174)-EGFP

   Plasmid

   #196232

   Purpose

   EGFP fused to the C-terminus of tandem WWE domains within Deltex2 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   DTX2(8-174)-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP and Myc tag

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP

   Plasmid

   #196235

   Purpose

   EGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   C-terminal EGFP tagged Af1521(K35E/Y145R)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K35E and Y145R

   Promoter

   EF1A

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-Hygro-EF1A-RNF146(100-182)-myc

    Plasmid

    #196236

    Purpose

    RNF146(100-182) WWE domain with a myc tag fused to the C terminus & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    RNF146(100-182) encoding a C-terminal myc tag
    

    Use

    Lentiviral

    Tags

    myc tag

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-Hygro-EF1A-RNF146(92-168)-EGFP

    Plasmid

    #196228

    Purpose

    EGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    RNF146(92-168)-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP and Myc tag

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. PABPC1_544-626_pGEX6P1

    Plasmid

    #209790

    Purpose

    Bacterial expression of PABPC1 Mlle domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al J Biol Chem. 2010 Apr 30;285(18):13599-6

    Insert

    Mlle domain of PABPC1 (PABPC1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 544-626 only

    Promoter

    tac

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMVQA

    Plasmid

    #197894

    Purpose

    pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

    Depositor

    Thomas Klimkait

    Article

    Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

    Insert

    HIV-1 3' end (HXB2: 9045 till poly-A(10)
    

    Expression

    Bacterial

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR4 Ta-SRO1 JN177

    Plasmid

    #192546

    Purpose

    pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR4 Ta-sro1 SR3

    Plasmid

    #192547

    Purpose

    pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACEBac1-PARP1-Flag-His-WT

    Plasmid

    #111572

    Purpose

    Expresses full length PARP1 in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-polyQ74-NLS

    Plasmid

    #231892

    Purpose

    Forms nuclear HTT partial exon 1 Q74-NLS aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74-NLS (HTT Human)
    

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. SaCas9 GFP V3

    Plasmid

    #226962

    Purpose

    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PDG459 V3

    Plasmid

    #226958

    Purpose

    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPINE GFP nanobody:Halo:His6

    Plasmid

    #111090

    Purpose

    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

    Depositor

    Lennart Wirthmueller

    Article

    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

    Insert

    GFP Nanobody
    

    Tags

    Halo and His6

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    T7 promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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