We narrowed to 2,372 results for: PUC19

1. pUC19-U6-Bs3-sgRNA (BsaI)

   Plasmid

   #188280

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

   Insert

   Bs3-sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Promoter

   U6

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19 KGA HA5'-mKO2-3'

   Plasmid

   #110406

   Purpose

   Homology donor for glutaminase KGA isoform knock-in, mKO2

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. puC19 preACT1(del6) short BP-3'SS

   Plasmid

   #111333

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Mutation

   shorter branch to 3' splice site distance

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. puC19 preACT1(del6) AcAc

   Plasmid

   #111332

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Mutation

   3' splice site AcAc

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pre-Ubc4 BrG pUC19

   Plasmid

   #111322

   Purpose

   for transcription to Ubc4 pre-mRNA

   Depositor

   Christine Guthrie

   Article

   Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

   Insert

   UBC4 (UBC4 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   intron has branch-site A to G mutation

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19 gene 60 thru gap

   Plasmid

   #49160

   Purpose

   Template for generating gene 60 mRNA truncated two codons after coding gap

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 60
   

   Mutation

   gene 60 mRNA truncated two codons after coding ga…

   Promoter

   T7

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19 gene 60 to gap + Cys

   Plasmid

   #49159

   Purpose

   Template for generating gene 60 mRNA, sequence 5' of coding gap + Cys

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 600
   

   Mutation

   Coding gap and downstream sequence have been dele…

   Promoter

   T7

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. puC19 preACT1(del6) A259C

   Plasmid

   #111329

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Mutation

   branch site A259C

   Availability

   Academic Institutions and Nonprofits only

   

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9. puC19 preACT1(del6) C256A

   Plasmid

   #111331

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Mutation

   branch site C256A

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-P70a-Top-T~P70a-Bottom-T

    Plasmid

    #87314

    Purpose

    Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19/PLIN2 GFP-P2A-BFP

    Plasmid

    #202195

    Purpose

    knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP-P2A-BFP knock-in

    Promoter

    lac

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-TgKI-MCS-tdTomato-polyA

    Plasmid

    #174022

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    tdTomato
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-dual-scafffold-2.0 (pBP49)

    Plasmid

    #218931

    Purpose

    The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Insert

    dual scaffolds for CRISPR
    

    Use

    CRISPR

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-eGFP-P2A-dTAG-Ing3

    Plasmid

    #170754

    Purpose

    Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

    Depositor

    Kristian Helin

    Article

    Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

    Insert

    eGFP-P2A-2xHA-dTAG
    

    Use

    Mouse Targeting

    Tags

    eGFP-P2A-2xHA-dTAG

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    none

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid

    #223137

    Purpose

    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIG-747pUC19-tNGFR-P2A-CD7

    Plasmid

    #186086

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD7 (CD7 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

    Plasmid

    #188634

    Purpose

    HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Human, Synthetic)
    

    Use

    CRISPR

    Tags

    CD3ε-mNeonGreen

    Expression

    Mammalian

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKB33 (pUC19-KANMX-tetO7-CYCpr)

    Plasmid

    #210472

    Purpose

    Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Insert

    KanMX-TetO7-CYCpr cassette
    

    Use

    Synthetic Biology

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIG-730pUC19-tNGFR-P2A-Coronin1A

    Plasmid

    #186110

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CORONIN1A
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIG-703pUC19-tNGFR-P2A-BACH2

    Plasmid

    #186112

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    BACH2 (BACH2 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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