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Addgene
Showing: 221 - 240 of 243 results

  1. pcDNA3-K-I-N-CAAX

    Plasmid
    #41640
    Purpose
    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Insert
    Dronpa145K-IntersectinDH-Dronpa145N-CAAX
    Use
    Tags
    CAAX box
    Expression
    Mammalian
    Mutation
    K145N on Dronpa
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    41640-plasmid-map-sequence-id-240702

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  12. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA

    Plasmid
    #165437
    Purpose
    Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-RhoA in mammalian cells as a negative control for FLIM imaging.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA
    Use
    Tags
    ShadowY, Clover(T153M/F223R)
    Expression
    Mammalian
    Mutation
    H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    165437-plasmid-map-sequence-id-325980

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  18. RHOU

    Plasmid
    #38885
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RHOU (RHOU Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C191L
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 221 - 240 of 243 results