We narrowed to 24,357 results for: nes

1. Flag-TurboID-NES_pLX304

   Plasmid

   #202008

   Purpose

   expresses TurboID in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   Flag-TurboID-NES
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEXP(V5-APEX2-NES)

   Plasmid

   #107596

   Purpose

   Expresses V5-APEX2-NES in mammalian cells

   Depositor

   Markus Hafner

   Article

   Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

   Insert

   APEX2-NES
   

   Use

   Frt site to insert into hek293 t-rex genome

   Tags

   NES and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Dox inducible

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. NES-EGFP-cPHx3

   Plasmid

   #116855

   Purpose

   PI(3,4)P2 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Use

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   amino acids 169-329 (tandem trimer)

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUAS-V5-miniTurboID-NES

   Plasmid

   #116905

   Purpose

   Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

   Depositor

   Norbert Perrimon

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurboID
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUAS-V5-TurboID-NES

   Plasmid

   #116904

   Purpose

   Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

   Depositor

   Norbert Perrimon

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PV-NES-GFP

   Plasmid

   #17301

   Depositor

   Anton Bennett

   Article

   Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

   Insert

   PV-NES-GFP (Pvalb Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. V5-GFP-APEX2-NES_pLX208

   Plasmid

   #202018

   Purpose

   expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-GFP-APEX2-NES
   

   Use

   Lentiviral

   Tags

   GFP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RANLS-DEVD-BNES

   Plasmid

   #50840

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A and ddRFP B
   

   Use

   Tags

   NES sequence LALKLAGLDIGS and triplicated NLS seq…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. GANLS-DEVD-BNES

   Plasmid

   #50835

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP A and ddRFP B
   

   Use

   Tags

   NES sequence LALKLAGLDIGS and triplicated NLS seq…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-Halotag-FRB-EX-nes_pLX304

    Plasmid

    #120913

    Purpose

    Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    HA-Halotag-FRB-EX-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. V5-LOV-Turbo-NES

    Plasmid

    #199545

    Purpose

    expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NES and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. NES-mCherry-P4Mx1

    Plasmid

    #108143

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mPA-GFP-Farnesyl-5

    Plasmid

    #57133

    Purpose

    Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. mCherry-Nesprin-1αKASH

    Plasmid

    #187015

    Purpose

    Disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Lombardi et al J Biol Chem. 2011 Jul 29;286(30):26743-5

    Insert

    Nesprin-1alphaKASH
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. NES-mCherry-cPHx3

    Plasmid

    #116862

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem trimer)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MMLVgag-3NES-Cre

    Plasmid

    #207252

    Purpose

    Generation of CreVLPs when used in conjunction with MMLV gag-pol during production

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    MMLVgag-3NES-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. m6A-Tracer-NES

    Plasmid

    #159607

    Purpose

    Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.

    Depositor

    Aaron Streets

    Article

    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

    Insert

    m6A-Tracer-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVITRO1-mCardinal-NES

    Plasmid

    #184453

    Purpose

    Cytosolic mCardinal expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    mCardinal-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-NES-KIRIN1

    Plasmid

    #107416

    Purpose

    Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1
    

    Use

    Tags

    Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO_NES_HaloTag9

    Plasmid

    #175537

    Purpose

    Strict cytosolic expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    NES-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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