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pHAGE-PTPN11-N308D

Plasmid

#116644

Purpose

Lentiviral expression of PTPN11 N308D

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTPN11 (PTPN11 Human)

Use

Lentiviral

Mutation

N308D

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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MIP 339 CoMiP coLin28 2A Nanog IRES eGFP

Plasmid

#63729

Purpose

To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

Depositor

Mark Kay, Joseph Wu

Article

Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

Inserts

Lin28 (LIN28A Human)
Nanog (NANOG Human)

Tags

T2A

Expression

Mammalian

Mutation

codon optimized

Promoter

SFFV

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223_CYLD_WT

Plasmid

#82326

Purpose

Gateway Donor vector containing CYLD , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYLD (CYLD Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RASA1_WT

Plasmid

#81778

Purpose

Gateway Donor vector containing RASA1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RASA1 (RASA1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcrDNA3.1-Chimera

Plasmid

#100891

Purpose

Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma

Depositor

Sanford Simon

Article

Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

Insert

Major Fibrolamellar hepatocellular carcinoma Chimera (DNAJB1, PRKACA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKACA

Plasmid

#23495

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKACA (PRKACA Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CDK4

Plasmid

#23778

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK4 (CDK4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-KHK

Plasmid

#23586

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

KHK (KHK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-ALPK2

Plasmid

#23493

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ALPK2 (ALPK2 Human)

Use

Gateway donor vector

Mutation

A117G

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pHAGE-PTPN11-Q79R

Plasmid

#116646

Purpose

Lentiviral expression of PTPN11 Q79R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTPN11 (PTPN11 Human)

Use

Lentiviral

Mutation

Q79R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-CDK5R1

Plasmid

#23779

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK5R1 (CDK5R1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CDK2

Plasmid

#20447

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CDK2 (CDK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Y276C

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTPN11-E76Q

Plasmid

#116642

Purpose

Lentiviral expression of PTPN11 E76Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTPN11 (PTPN11 Human)

Use

Lentiviral

Mutation

E76Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK15

Plasmid

#23697

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK15 (MAPK15 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CDK4

Plasmid

#20448

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223_RASA1_p.L146S

Plasmid

#81606

Purpose

Gateway Donor vector containing RASA1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RASA1 (RASA1 Human)

Use

Gateway entry vector

Mutation

L146S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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delta-BRIP1-pcDNA3.1/MycHis

Plasmid

#19042

Depositor

Ronny Drapkin

Article

De Nicolo et al Clin Cancer Res. 2008 Jul 15. 14(14):467

Insert

delta BRIP1 (BRIP1, BRCA1 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

This is a novel BRIP1 germline mutation that cons…

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CDK5

Plasmid

#20449

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CDK5 (CDK5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-MAK

Plasmid

#23624

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAK (MAK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pHAGE-PTPN11-G60R

Plasmid

#116643

Purpose

Lentiviral expression of PTPN11 G60R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTPN11 (PTPN11 Human)

Use

Lentiviral

Mutation

G60R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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