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YIplac128-TEF2p-LDH-CYC1t

Plasmid

#216120

Purpose

Yeast integrative vector for constitutive expression of LDH at the LEU2 locus

Depositor

Yvonne Nygård

Article

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Lactate dehydrogenase (LDH Bovine)

Expression

Yeast

Promoter

TEF2 promoter

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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YIplac204-TDH3p-LDH-CYC1t

Plasmid

#216121

Purpose

Yeast integrative vector for constitutive expression of LDH at the TRP1 locus

Depositor

Yvonne Nygård

Article

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Lactate dehydrogenase (LDH Bovine, Bos taurus)

Expression

Yeast

Promoter

TDH3 promoter

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13239

Plasmid

#219912

Purpose

The base plasmid of TUNEYALI for TF49

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49

Expression

Yeast

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRRv2_sgTom

Plasmid

#218346

Purpose

CRISPR KO plasmid for tdTomato in human cell lines

Depositor

Seth Parker

Article

Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

Insert

Inserted sgRNA targeting tdTomato

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13247

Plasmid

#219918

Purpose

The base plasmid of TUNEYALI for TF57

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13228

Plasmid

#219903

Purpose

The base plasmid of TUNEYALI for TF38

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF38 (YALI1_A14345g) and homologous arm matching TF38

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13210

Plasmid

#219885

Purpose

The base plasmid of TUNEYALI for TF20

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pACB108

Plasmid

#222207

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-TASL

Plasmid

#221265

Purpose

Expression of TASL in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TASL (Tasl Mouse)

Use

Retroviral

Tags

3xFLAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-ZC3H12B

Plasmid

#221300

Purpose

Expression of ZC3H12B (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ZC3H12B (pLxIS)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13220

Plasmid

#219895

Purpose

The base plasmid of TUNEYALI for TF30

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30

Expression

Yeast

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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CMV DJ-1 C106A

Plasmid

#220914

Purpose

Expresses DJ-1 with the C106A mutation

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

DJ-1 (PARK7 Human)

Expression

Mammalian

Mutation

C106A

Promoter

CMV

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12622

Plasmid

#219929

Purpose

PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter ACT

Expression

Yeast

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13237

Plasmid

#219910

Purpose

The base plasmid of TUNEYALI for TF47

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF47 (YALI1_C19151g) and homologous arm matching TF47

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12562

Plasmid

#219923

Purpose

PrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter GPD

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13186

Plasmid

#219924

Purpose

PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter TEF

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13204

Plasmid

#219879

Purpose

The base plasmid of TUNEYALI for TF14

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF14 (YALI1_F21261g) and homologous arm matching TF14

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13200

Plasmid

#219875

Purpose

The base plasmid of TUNEYALI forTF10

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF10 ( YALI1_C16204g) and homologous arm matching TF10

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13242

Plasmid

#219914

Purpose

The base plasmid of TUNEYALI for TF52

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF52 (YALI1_E12668g) and homologous arm matching TF52

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13245

Plasmid

#219916

Purpose

The base plasmid of TUNEYALI for TF55

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF55 (YALI1_A16891g) and homologous arm matching TF55

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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