We narrowed to 36,291 results for: lat
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Plasmid#228633PurposeBacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)DepositorInsertEnterovirus Coxsackievirus A71 2A protease
TagsHis-SUMOExpressionBacterialMutationC110AAvailable sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag)
Plasmid#228661PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Zika virus for biochemical assayDepositorInsertZika virus portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)
Plasmid#217803PurposeVariant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag
Plasmid#228635PurposeBacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interactionDepositorInsertEnterovirus A71 3C protease isoform A
TagsHis-AviExpressionBacterialAvailable sinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag
Plasmid#228634PurposeBacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interactionDepositorInsertEnterovirus Coxsackievirus A71 2A protease
TagsHis-AviExpressionBacterialAvailable sinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library
Pooled library#138015PurposeAAV library targeting transcriptional regulators expressed in cardiomyocytes.DepositorSpeciesMus musculusUseAAV, CRISPR, and Cre/LoxAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)
Plasmid#217797PurposeVariant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.DepositorInsertCdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationCdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
FNL Combinatorial Cloning Platform
Plasmid kit#1000000211PurposeA Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery.DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector typeBacterial Expression, Insect Expression, Mammalian ExpressionCloning typeMultiSite GatewayAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
EMMA toolkit
Plasmid kit#1000000119PurposeCollection of Golden Gate vectors and standardized genetic parts for the construction mammalian expression plasmids.DepositorApplicationCloning and Synthetic BiologyVector typeMammalian ExpressionCloning typeGolden GateAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mouse Cherry Brie Pooled Library
Pooled library#170511PurposeGenome-wide knockout library. It is identical to the Brie library #73633, replacing Puromycin resistance with mCherry.DepositorPublicationExpressionMammalianSpeciesSyntheticUseCRISPR and LentiviralAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Inzolia library in pRDA_550
Pooled library#209552PurposeThe human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.DepositorUseCRISPR and LentiviralAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs ABCDE>A
Plasmid#83318Purposehuman DNA-PKcs/ABCDE>alaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman DNA-PKcs (PRKDC Human)
ExpressionMammalianMutation6 phosphorylation sites in the ABCDE cluster subs…Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
T7-RelA(S536E)
Plasmid#24156DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRelA (RELA Human)
TagsT7 gp10ExpressionMammalianMutationS536EAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human Lipid Droplet and Metabolism Library
Pooled library#191535PurposeTargets 1,196 genes related to lipid droplet biology and cell metabolism.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSMAL-CellTag-multi-v1 barcode library
Pooled library#206045PurposeThe CellTag multi library of barcodes can be used to clonally label cells with DNA barcodes. These barcodes can be profiled directly with single-cell RNA and ATAC sequencing, to allow clonal trackingDepositorUseLentiviralAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs PQR>ala
Plasmid#83319Purposehuman DNA-PKcs PQR>alaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman DNA-PKcs (PRKDC Human)
ExpressionMammalianMutation5 phosphorylation sites in the PQR cluster substi…Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX1
Plasmid#46848PurposeVector modified from pBluescript SK(+) by adding more cloning sitesDepositorTypeEmpty backboneUseCloning vectorAvailable sinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMKO.1-Puro-shPP2A-Aalpha
Plasmid#15249DepositorPublicationInsertPP2A regulatory subunit A, alpha isoform (PPP2R1A Human)
UseRNAi and RetroviralExpressionMammalianAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER-21-RUNX1-P2A-ERG
Plasmid#97045PurposeExpresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.DepositorInsertRunt Related Transcription Factor 1, ETS-Related Gene
UseLentiviralExpressionMammalianPromoterTet onAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
hFLT1
Plasmid#83435Purposehuman VEGFR1 with HA and FLAG tagDepositorInserthuman VEGFR1 (FLT1 Human)
TagsFlag and MycExpressionMammalianMutationHA-tag was inserted 30 AA downstream of the FLT1 …PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only