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pFE-sfGFP

Plasmid

#132998

Purpose

pFE carrying a sfGFP gene

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

sfGFP

Tags

6xhis

Expression

Bacterial

Promoter

tetR

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX-mTurquoise2

Plasmid

#138390

Purpose

RMCE vector for generating C-terminal mTurqouise2 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

mTurquoise2

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pFE-dabA2

Plasmid

#133001

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

dabA2

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabB2

Plasmid

#133000

Purpose

pFE carrying a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

dabA2

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLR5-U6-sgRNA-EF

Plasmid

#82635

Purpose

piggyBac vector for expressing modified sgRNA in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Type

Empty backbone

Use

CRISPR; Piggybac vector

Expression

Mammalian

Promoter

U6

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ AMIC

Plasmid

#118420

Purpose

For mammalian expression of Amoeba myosin 1C

Depositor

Michael Tsang

Article

Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

Insert

Amoeba myosin 1C

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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IgH intron enhancer

Plasmid

#127247

Purpose

To maximize gene expression in B cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

heavy chain enhancer

Expression

Mammalian

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI119

Plasmid

#126059

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

chloroplastic FNR transit peptide

Use

Synthetic Biology; Module 0

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI120

Plasmid

#126060

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Synthetic Biology; Module 0

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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ARK2a

Plasmid

#81017

Purpose

E.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Inserts

MevTo-BBa1002-pTrc-Mkco
PMD

Promoter

LacUV5 and Trc promoter

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Xlim-1 (Lhx1) 3m

Plasmid

#74136

Purpose

This is a mutant of Xlim-1/Lhx1 with one point mutation in each of the two LIM domains. Cloned in XbetaM. To prepare sense RNA: SalI/SP6.

Depositor

Igor Dawid

Article

Taira et al Nature. 1994 Dec 15;372(6507):677-9.

Insert

lhx1 3m

Use

Expression in frog embryo

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pCMB-TGNVHAr

Plasmid

#61179

Purpose

Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtVHA-a1

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-GAr

Plasmid

#61173

Purpose

Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

GmMAN49-mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-MITr

Plasmid

#61199

Purpose

Fluorescent reporter for motochondria ScCOX4sp-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

ScCOX4sp-mCherry

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-TGN41r

Plasmid

#61177

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP41

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-LEr

Plasmid

#61186

Purpose

Fluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB5A2

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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RASL12

Plasmid

#55679

Purpose

expression of mouse RASL12

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASL12 (Rasl12 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pLL3.7 Alk.2 shRNA

Plasmid

#59297

Purpose

shRNA to mouse Alk

Depositor

Ulrike Heberlein

Article

Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

Insert

Alk.2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pFS320-USP44-catDead

Plasmid

#43901

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Tags

MYC

Expression

Mammalian

Mutation

C282A, T91A

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pFS322-USP44-catDeadMyc

Plasmid

#43904

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Tags

HIS and MYC

Expression

Mammalian

Mutation

C282A, T91A

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only