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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,151 results for: nes

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Showing: 2401 - 2420 of 17151 results

  1. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Publication
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    80424-plasmid-map-sequence-id-151441

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  7. pGolt-mCherry / pGolt3-mCherry

    Plasmid
    #73297
    Purpose
    Visualization of Dendritic Golgi Satellite
    Depositor
    Michael Kreutz
    Publication
    Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    Tags
    ER export and mCherry
    Expression
    Mammalian
    Promoter
    CMV immediate early promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73297-plasmid-map-sequence-id-135341

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  10. pNICKclos2.0

    Plasmid
    #73228
    Purpose
    Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052
    Depositor
    Sheng Yang
    Publication
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9 nickase
    sgRNA to xylR
    Use
    E.coli - clostridium shuttle vector
    Mutation
    D10A
    Promoter
    Pj23119 and Pthl
    Available since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73228-plasmid-map-sequence-id-178086

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  20. All_in_one_CRISPR/Cas9_LacZ

    Plasmid
    #74293
    Purpose
    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening
    Depositor
    Lynne Postovit
    Publication
    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LacZ-alpha
    Cas9
    mCherry
    Use
    CRISPR
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    Lac promoter, SV40, and T7 promoter
    Available since
    May 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    74293-plasmid-map-sequence-id-140421

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Showing: 2401 - 2420 of 17151 results