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pDONR223_PIK3R3_WT

Plasmid

#81903

Purpose

Gateway Donor vector containing PIK3R3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway entry vector

Mutation

D338A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pNOC_dfnCas12a-KRAB_crRNA NC

Plasmid

#176262

Purpose

pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

Kruppel associated box domain and luciferase

Mutation

D917A and E1006A mutations to inactivate the endo…

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Ubxd8

Plasmid

#123599

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Fas associated factor family member 2 (Faf2 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.K101I

Plasmid

#81641

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Mutation

K101I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.W131C

Plasmid

#81643

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Mutation

W131C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.E47A

Plasmid

#81645

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Mutation

E47A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2

Plasmid

#173952

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_XIAP_WT

Plasmid

#82170

Purpose

Gateway Donor vector containing XIAP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

XIAP (XIAP Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-ephrinB1-SV40-RFP (b1R)

Plasmid

#65446

Purpose

Expression of ephrinB1 and RFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

ephrin B1 (Efnb1 Mouse)

Use

Lentiviral

Tags

SV40-RFP

Expression

Mammalian

Promoter

UBQ

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223_SPOP_p.K134N

Plasmid

#81647

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Mutation

K134N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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