We narrowed to 3,135 results for: actin

1. pET28a-Hook3 D102A, E108A

   Plasmid

   #74618

   Purpose

   Expresses a double mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   D102A, E108A

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-Hook3 N68A, W69A, K77A

   Plasmid

   #74619

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-HP1alpha

   Plasmid

   #179703

   Purpose

   visualization of centromere heterochromatin

   Depositor

   Kenji Sugimoto

   Article

   Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

   Insert

   heterochromatin protein alpha (CBX5 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MIC60-mCherry

   Plasmid

   #232789

   Purpose

   Encodes an mCherry-tagged MIC60/IMMT

   Depositor

   Giulia Bertolin

   Article

   Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

   Insert

   MICOS complex subunit MIC60 (IMMT Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IPU-GFP-MYH9

   Plasmid

   #168273

   Purpose

   Expresses MYH9 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   MYH9 (MYH9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGM202T7

   Plasmid

   #69993

   Purpose

   E. coli/Streptomyces expression vector containing the T7-promoter

   Depositor

   Günther Muth

   Article

   Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

   Inserts

   aphII
   tsr
   rep
   sso
   T7 promoter
   dso
   His-Orf
   

   Use

   Tags

   N-terminal and C-terminal His tag encoding sequen…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. tetO-MITF

   Plasmid

   #170695

   Purpose

   doxycycline-inducible overexpression of MITF

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   MITF (MITF Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. tetO-SOX9

   Plasmid

   #170703

   Purpose

   doxycycline-inducible overexpression of SOX9

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   SOX9 (SOX9 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mouse E-cadherin GFP (423)

   Plasmid

   #67937

   Purpose

   Mammalian expression of E-cadherin-GFP

   Depositor

   Alpha Yap

   Article

   Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

   Insert

   E-cadherin (Cdh1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX neo ACTN1-GFP

    Plasmid

    #138293

    Purpose

    Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    ACTN1 (ACTN1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBADHISTEV_PfeE

    Plasmid

    #129120

    Purpose

    pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

    Depositor

    James Naismith

    Article

    Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

    Insert

    Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    PBAD

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

    Plasmid

    #176542

    Purpose

    Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Inserts

    MBP tag
    TnpB
    

    Use

    Tags

    3' extension derived from endogenous locus a…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-hIP-dCas9-BSD

    Plasmid

    #183231

    Purpose

    Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Human insulin promoter

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9-beta-mmr

    Plasmid

    #169241

    Purpose

    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    mutL-E36K
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed glutamic acid 36 to lysine

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA Myc-mMEKK2

    Plasmid

    #44158

    Depositor

    Xin Lin

    Article

    Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

    Insert

    Mekk2 (Map3k2 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

16. tetO-MESP1

    Plasmid

    #170694

    Purpose

    doxycycline-inducible overexpression of MESP1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    MESP1 (MESP1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAL130

    Plasmid

    #22475

    Purpose

    Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF3NT (PIF3 Mustard Weed, Mouse)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 Myc-huMEKK3 (K391A)

    Plasmid

    #44157

    Depositor

    Xin Lin

    Article

    Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

    Insert

    MEKK3 (K391A) (MAP3K3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K391A, kinase-dead mutant

    Promoter

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

19. GST-PBD H538A/K540M

    Plasmid

    #60879

    Purpose

    Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA 365-653 H538A/K540M mutant

    Depositor

    Trina Schroer

    Article

    Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1

    Insert

    Polo-like kinase 1 (PLK1 Human, BC01486)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    H538A/K540M

    Promoter

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

20. His-ZZ-TEV-BICDR1

    Plasmid

    #111859

    Purpose

    Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1 (Bicdl1 Mouse)
    

    Use

    Tags

    His-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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