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pBEL2636

Plasmid

#232500

Purpose

Expression plasmid of of LetA with LetB(F468B)

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetB F468Amber

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4b PhiC31 attP41

Plasmid

#242881

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA AAV 4a PhiC31 attP41

Plasmid

#242882

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA AAV 3b PhiC31 attP41

Plasmid

#242883

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4b Bxb1 attB38

Plasmid

#242879

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for Bxb1 attB site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4a Bxb1 attB38

Plasmid

#242878

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for Bxb1 attB site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4a PhiC31 attP41

Plasmid

#242880

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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B8-pVTCB052

Plasmid

#231302

Purpose

Plasmid used for expression of AI-designed GFP protein B8 in E. coli

Depositor

Alexander Rives

Article

Hayes et al Science. 2025 Feb 21;387(6736):850-858.

Insert

B8

Expression

Bacterial

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pPglL-strep-mClover3

Plasmid

#196665

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

PglL

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Promoter

araBAD

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-strep-mClover3

Plasmid

#196668

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Promoter

araBAD

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

Plasmid

#208869

Purpose

For mammalian expression of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

HA

Expression

Mammalian

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-GST-TEV-GFP-NAP1

Plasmid

#208870

Purpose

For expression in E. coli of GFP-NAP1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

GFP and GST-TEV

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-LaM4-2xTS

Plasmid

#162779

Purpose

Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Article

Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

Insert

anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope

Tags

BAP, HA, His6, T7, and TS site from proCCK

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2196

Plasmid

#170400

Purpose

For expresson of LetA-[LetB PLL6->1] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ702-717, replaced with 114-130)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2197

Plasmid

#170401

Purpose

For expresson of LetA-[LetB PLL6->3] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ702-716, replace with 347-360)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2198

Plasmid

#170402

Purpose

For expresson of LetA-[LetB PLL6->5] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ702-716, replace with 580-600)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2251

Plasmid

#170403

Purpose

For expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 225-249)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2252

Plasmid

#170404

Purpose

For expresson of LetA-[LetB PLL6->4] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ700-716, replace with 455-479)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2253

Plasmid

#170405

Purpose

For expresson of LetA-[LetB PLL6->7] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 812-836)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-TOMM20-dTomato

Plasmid

#115922

Purpose

donor to insert dTomato at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-dTomato

Use

Donor template

Tags

dTomato

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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