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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 2461 - 2480 of 9221 results

  2. pSECRETS-B

    Plasmid
    #196987
    Purpose
    For SECRETS protocol to screen for gRNA activity and specificity: BsaI cassette for gRNA expression or for cloning (x-)gRNA librarys + off-target for SECRETS counter-selection.
    Depositor
    Eric Josephs
    Article
    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1
    Insert
    Spy gRNA cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pltetO1
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    196987-plasmid-map-sequence-id-389302

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  3. pBTK1061

    Plasmid
    #209550
    Purpose
    BTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA gene
    Depositor
    Jeffrey Barrick
    Article
    Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.
    Insert
    E2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
    Use
    Synthetic Biology
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    209550-plasmid-map-sequence-id-418282

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Showing: 2461 - 2480 of 9221 results