We narrowed to 4,258 results for: biorxiv

1. pAG415Gal_Gcn4 SATtoG KtoR

   Plasmid

   #232961

   Purpose

   Galactose iduced expression of Gcn4 ATtoG KtoR in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 SATtoG KtoR
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAG415Gal_Gcn4 ILVtoED solvvol

   Plasmid

   #232958

   Purpose

   Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED solvvol
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG415Gal_Gcn4 SATtoG KtoRGFP

   Plasmid

   #233006

   Purpose

   Galactose iduced expression of Gcn4 SATtoG KtoRGFP in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 SATtoG KtoR
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG415Gal_Gcn4 solvvol W

   Plasmid

   #232977

   Purpose

   Galactose iduced expression of Gcn4 solvvol W+ in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol W+
   

   Use

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIVT-Bxb1-I87L, A369P, E434G

   Plasmid

   #232750

   Purpose

   IVT template for making modRNA encoding mutant Bxb1modRNA (I87L, A369P, E434G)

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   I87L, A369P, E434G

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINF AtMKK5

   Plasmid

   #228396

   Purpose

   Recombinant expression of His6-tagged Arabidopsis thaliana MKK5 in E. coli

   Depositor

   Lennart Wirthmueller

   Article

   Simranjit et al bioRxiv 2023.09.25.558804

   Insert

   MKK5 (MKK5 Mustard Weed)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210734

   Purpose

   Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Frodo sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)

   Plasmid

   #232128

   Purpose

   A middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA2 (GATA2 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

   Plasmid

   #232123

   Purpose

   A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

    Plasmid

    #232131

    Purpose

    A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    FOXF2 (FOXF2 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

    Plasmid

    #232130

    Purpose

    A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAV-CAG-oScarlet-WPRE-BC10-pA

    Plasmid

    #220774

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al bioRxiv 2024.04.24.590975

    Insert

    oScarlert-WPRE-BC10
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV-CAG-oScarlet-WPRE-BC7-pA

    Plasmid

    #220771

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al bioRxiv 2024.04.24.590975

    Insert

    oScarlert-WPRE-BC7
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTBL3342 PB-PEmax-puro

    Plasmid

    #226674

    Purpose

    PiggyBac cargo vector encoding PEmax and puromycin resistance.

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    PEmax
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SUPV3L1-HA WT

    Plasmid

    #232348

    Purpose

    Expresses human SUPV3L1/SUV3 in mammalian cells with HA tag

    Depositor

    Samantha Lewis

    Article

    Begeman et al bioRxiv [Preprint]. 2024 Aug 17:2024.08.

    Insert

    SUPV3L1 (SUPV3L1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SUPV3L1-HA G207V

    Plasmid

    #232349

    Purpose

    Expresses human SUPV3L1/SUV3 (G207V) in mammalian cells with HA tag

    Depositor

    Samantha Lewis

    Article

    Begeman et al bioRxiv [Preprint]. 2024 Aug 17:2024.08.

    Insert

    SUPV3L1 (SUPV3L1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G207V

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Bxb1-GA_CAG-rtTA-T2A-mTagBFP2

    Plasmid

    #229797

    Purpose

    Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCas9-EcRT-Z3-Nat

    Plasmid

    #232094

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Bxb1-GA_AIO-TetOn_mScarlet_Convergent

    Plasmid

    #229790

    Purpose

    Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDest17_Gcn4 ILVtoED solvvol

    Plasmid

    #231858

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvol

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED solvvol
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    T7

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge