We narrowed to 2,328 results for: dap

1. pX330-PITCh-TUBB4B

   Plasmid

   #207785

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9-omega

   Plasmid

   #114632

   Purpose

   Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

   Depositor

   Anushree Chatterjee

   Article

   Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

   Insert

   dCas9-omega
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet promoter

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUASt-GBP-Trbo

   Plasmid

   #179996

   Purpose

   Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.

   Depositor

   Graydon Gonsalvez

   Article

   Baker et al Development. 2021 Nov 15;148(22). pii: 2

   Insert

   GBP nanobody
   

   Use

   Tags

   Trbo

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. -4200GAMT-P1-pGL4

   Plasmid

   #26804

   Depositor

   Sam Lee

   Article

   Ide et al Mol Cell. 2009 Nov 13. 36(3):379-92.

   Insert

   Guanidinoacetate N-methyltransferase (GAMT Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

   Plasmid

   #129774

   Purpose

   Marker of microtubule dynamics in C. elegans hypodermis

   Depositor

   Andrew Chisholm

   Article

   Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

   Inserts

   Pcol-19
   ebp-2 (ebp-2 Nematode)
   unc-54 3'UTR
   

   Use

   Synthetic Biology; Gateway

   Tags

   Synthetic Intron and mGFP

   Expression

   Worm

   Mutation

   Promoter

   Pcol-19

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-Hook3 aa 1-160-GCN4

   Plasmid

   #74608

   Purpose

   Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-160 (HOOK3 Human)
   

   Use

   Tags

   6x His, GCN4, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-JNKAR1-NES

   Plasmid

   #84632

   Purpose

   Unimolecular, FRET-based JNK activity reporter; cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   ECFP-FHA1-JNKsubDD-Citrine-NES
   

   Use

   Tags

   6xHis, Citrine, ECFP, Nuclear export signal (NES)…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Cerulean-WW-NES

   Plasmid

   #84629

   Purpose

   Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   Cerulean-WW-NES
   

   Use

   Tags

   6xHis, Cerulean, Nuclear export signal (NES), T7 …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMKO.1-shMki67

   Plasmid

   #160958

   Purpose

   Mki67 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Mki67 (Mki67 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6M-Pak1 P13A

    Plasmid

    #12207

    Depositor

    Jonathan Chernoff

    Article

    Galisteo et al J Biol Chem. 1996 Aug 30. 271(35):20997-

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P13A: disrupts first polyproline region of Pak1

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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