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pET21-pelB-LaTdT-9

Plasmid

#228977

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrU6-2-35S

Plasmid

#226706

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from CaMV 35S promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrU6-2

Use

CRISPR

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrActin-35S

Plasmid

#226708

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrActin

Use

CRISPR

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrActin-UBI

Plasmid

#226709

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrActin

Use

CRISPR

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrU6-2-UBI

Plasmid

#226707

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrU6-2

Use

CRISPR

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2B

Plasmid

#225069

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2B)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS9

Plasmid

#218717

Purpose

Expresses N-terminally EGFP-tagged BBS9 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS9 (BBS9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

A455T (g1876a)

Promoter

CMV

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT38

Plasmid

#218723

Purpose

Expresses N-terminally EGFP-tagged IFT38 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT38 (CLUAP1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b-effector-ncRNA-rrt-His8

Plasmid

#194089

Purpose

Expresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coli

Depositor

Tingting Zou

Article

Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

Insert

Ec86 effector-ncRNA-rrt

Tags

His8

Promoter

T7

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pI pm

Plasmid

#218098

Purpose

Phagemid that expresses LacI, and pI under the Lac promoter

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Insert

pI

Expression

Bacterial

Promoter

Lac promoter

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Hs4EBP3_36-69-L61A V67A-GB1_AB

Plasmid

#148380

Purpose

Bacterial Expression of Hs4EBP3_36-69-L61AV67A

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP3_36-69-L61AV67A (EIF4EBP3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Hs4EBP3_36-69-Y40AL45A-GB1_AA

Plasmid

#148280

Purpose

Bacterial Expression of Hs4EBP3_36-69-Y40AL45A

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP3_36-69-Y40AL45A (EIF4EBP3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Hs4EBP3_36-69-YLLVtoAAA-GB1_AA

Plasmid

#148281

Purpose

Bacterial Expression of Hs4EBP3_36-69-YLLVtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP3_36-69-YLLVtoAAAA (EIF4EBP3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-F41DW42D_AB

Plasmid

#148372

Purpose

Insect Expression of Dm4E-T-F41DW42D

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T-F41DW42D (4E-T Fly)

Expression

Insect

Mutation

5 non silent mutation L127S, E493G, H628R, N659S,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-YLFWtoAADD_AB

Plasmid

#148373

Purpose

Insect Expression of Dm4E-T-YLFWtoAADD

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T-YLFWtoAADD (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations H611R and N659S and 8 si…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_32-83-GB1_AA

Plasmid

#148267

Purpose

Bacterial Expression of DmThor_32-83

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_32-83 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pKI-cjT-tdTomato-PGK-Neo

Plasmid

#186173

Purpose

Knock-in gene targeting in marmoset cells at TBXT locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjOSR1-EGFP-PGK-Puro

Plasmid

#186170

Purpose

Knock-in gene targeting in marmoset cells at OSR1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

EGFP

Use

Marmoset targeting

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-tdTomato-PGK-Neo

Plasmid

#186172

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-hCD271-PGK-Neo

Plasmid

#186171

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

hCD271

Use

Marmoset targeting

Mutation

human codon-optimized

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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