We narrowed to 150,454 results for: ins

1. pDonor_RB-TnV2

   Plasmid

   #213907

   Purpose

   Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB078d5

   Plasmid

   #79204

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ppro1 434cI
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAB094a

   Plasmid

   #79241

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PBAD rpoZ-HA4; PC araC
   

   Expression

   Bacterial

   Promoter

   PBAD; PC

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS178

   Plasmid

   #215678

   Purpose

   Empty repair plasmid for ChrII split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + MCS + loxP + rps-0p::5'ΔHygR
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   5' ∆HYGR encodes aa1-226

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHACK-GAL4>QF2(newHA1)

   Plasmid

   #104873

   Purpose

   HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   T2A-QF2
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p6610 MSCV-IP N-HAonly TIMP1

   Plasmid

   #35009

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   TIMP1 (TIMP1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

7. p6592 MSCV-IP N-HAonly WWP2

   Plasmid

   #34905

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   WWP2 (WWP2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

8. DT12A

   Plasmid

   #80416

   Purpose

   Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

   Depositor

   Thomas Cooper

   Article

   Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

   Insert

   human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPI138

   Plasmid

   #113229

   Purpose

   extrachromosomal expression vector, expression of LifeAct-GFP

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   LifeAct
   

   Use

   Dictyostelium discoideum expression vector, extra…

   Tags

   GFP

   Promoter

   act15

   Available Since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCD-Ugi22

    Plasmid

    #207004

    Purpose

    pGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)

    Depositor

    Wayne Parrott

    Article

    Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

    Insert

    Utricularia gibba insulator 22
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Nov. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCD-Ugi21

    Plasmid

    #207005

    Purpose

    pGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)

    Depositor

    Wayne Parrott

    Article

    Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

    Insert

    Utricularia gibba insulator 21
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Nov. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. RIP-Timer (DM#285)

    Plasmid

    #15109

    Depositor

    Douglas Melton

    Article

    Dor et al Nature. 2004 May 6. 429(6987):41-6.

    Insert

    insulin II promoter (Ins2 Rat)
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-EGFP-C1-HsAGO1_F

    Plasmid

    #146314

    Purpose

    Mammalian Expression of HsAGO1

    Depositor

    Elisa Izaurralde

    Article

    Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

    Insert

    HsAGO1 (AGO1 Human)
    

    Expression

    Mammalian

    Mutation

    N574S

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDV5 containing Sb-convex

    Plasmid

    #132696

    Purpose

    pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Sb_convex including 5' and 3' flanking sequences used for ribosome display
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS406-PHO5-GFP-hFAPP1 PH domain

    Plasmid

    #58723

    Purpose

    Expresses GFP-Tagged human FAPP1 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

    Insert

    Four-phosphate-adaptor protein 1 PH domain (PLEKHA3 Human)
    

    Tags

    GFP and Myc

    Expression

    Yeast

    Promoter

    PHO5

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-Flag-Tet3 CD Mut

    Plasmid

    #72222

    Purpose

    Express flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 3
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET3 catalytic domain (cata…

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

17. YSBE 508 : pNEO_GFP

    Plasmid

    #92081

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, Neo selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    GFP
    

    Use

    tagging proteins in cryptococcus neoforman

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV_Lyn11-mTurquoise2-iLID

    Plasmid

    #161000

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    Lyn11-mTurquoise2-iLID
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOCC154

    Plasmid

    #118902

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. p6642 MSCV-P C-FlagHA 6bE7-Kozak

    Plasmid

    #35020

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV6b E7 (E7 HPV 6b)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    March 6, 2012

    Availability

    Academic Institutions and Nonprofits only