Addgene

We narrowed to 10,852 results for: kars;

Showing: 3751 - 3780 of 10852 results

Results per page:

Sort results by:

pCF1148

Plasmid

#222330

Purpose

Fluorescent reporter plasmid for Veillonella

Depositor

J Christopher Fenno

Article

Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

Insert

bs2 gene with Veillonella parvula SKV38 mdh promoter

Use

E. coli-veillonella shuttle plasmid

Tags

Expression

Bacterial

Mutation

Promoter

Veillonella parvula SKV38 mdh promoter

Available since

Oct. 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

Plasmid

#126216

Purpose

Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCOLA-Gent-EM7-Erv1p-PDI

Plasmid

#202486

Purpose

Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

EM7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_iETV2_P2A_GFP_Puro

Plasmid

#168805

Purpose

PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ETV2 (ETV2 Synthetic, Human)

Use

Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEXtev-HMG54kd

Plasmid

#26030

Depositor

Johann Deisenhofer

Article

Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

Insert

3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Catalytic domain (amino acids 426-488) Methion…

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST2-GST-TEV-OPTN

Plasmid

#198428

Purpose

Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

optineurin (OPTN Human)

Use

Tags

GST and TEV

Expression

Bacterial

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_SOX10_P2A_Hygro_Barcode

Plasmid

#120479

Purpose

Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX10 (SOX10 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_MYCN_P2A_Hygro_Barcode

Plasmid

#120463

Purpose

Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYCN (MYCN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGK-HELLO LV

Plasmid

#202001

Purpose

Expresses HELLO neoantigen in tumor cells

Depositor

Nikhil Joshi

Article

Cui et al Cell. 2021 Dec 9;184(25):6101-6118.e13.

Insert

HELLO

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

pGK

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-dual-CRISPR-U6-H1(pBP43)

Plasmid

#218930

Purpose

The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

Depositor

Baoxu Pang

Article

Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6 and H1

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1a-mMLH1dn

Plasmid

#174825

Purpose

Mammalian expression of mouse MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (codon optimized)

Use

Tags

Expression

Mammalian

Mutation

deletion of residues 758-760

Promoter

EF1a

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

Plasmid

#101139

Purpose

Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCOLA-Gent-EM7-Erv1p-DsbC

Plasmid

#202485

Purpose

Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

EM7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_MYCL_P2A_Hygro_Barcode

Plasmid

#120462

Purpose

Barcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYCL (MYCL Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
aeBlue chromoprotein

Plasmid

#117846

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, aeBlue

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT121_GalL_nCDA1-VQRBE3

Plasmid

#145086

Purpose

Expressing base editor nCDA1-VQRBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-VQRBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A, D1135V, R1335Q, T1337R)

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUHD.NFATc1nuc

Plasmid

#40616

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1nuc (NFATC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Promoter

CMV2

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
tsPurple chromoprotein

Plasmid

#117848

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_EF1a-DjCas13d-msfGFP-Blast

Plasmid

#226008

Purpose

DjCas13d protein for RNA targeting in piggyBac backbone

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Insert

DjCas13d RNA-targeting nuclease

Use

CRISPR

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
fwYellow chromoprotein

Plasmid

#117841

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, fwYellow

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SIN40C.SFFV.dTomato.let-7c

Plasmid

#169301

Purpose

Constitutive overexpression of let-7c with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

let-7c (MIRLET7C Human)

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
psicheck2 PTEN 3'UTR

Plasmid

#50936

Purpose

reporter for PTEN RNAi

Depositor

Pier Pandolfi

Article

Tay et al Cell. 2011 Oct 14;147(2):344-57. doi: 10

Insert

PTEN 3'UTR (PTEN Human)

Use

RNAi

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Promoter

T7

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
eforRed chromoprotein

Plasmid

#117837

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, eforRed

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIC-myc-GBAi W211A

Plasmid

#171754

Purpose

For the mammalian expression of myc-GBAi W211A

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PME-1 pcDNA-StrepIII

Plasmid

#119292

Purpose

Mammalian overexpression of StrepII-tagged full length PPME1

Depositor

Jukka Westermarck

Article

Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

Insert

PPME1 (PPME1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0317 HvsCas7-11 mammalian expression

Plasmid

#172511

Purpose

Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

HvsCas7-11 mammalian expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor

Plasmid

#184951

Purpose

Donor for both PiggyBac and Cp36 Recombinase

Depositor

Lacra Bintu, Patrick Hsu, Ami Bhatt, Michael Bassik

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

PuroR, P2A, mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CPSF5

Plasmid

#155480

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF5 (NUDT21 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Flag-hOct4

Plasmid

#20072

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hOct4 (POU5F1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIP-BSR-GFP11

Plasmid

#68716

Purpose

Split GFP assay

Depositor

Yutaka Hata

Article

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

bsr

Use

Retroviral

Tags

FLAG and GFP11

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge