We narrowed to 6,294 results for: Pet2

1. BPV00605 pET21GG2-His(6)-BECN(1-265)

   Plasmid

   #136686

   Purpose

   Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 IDR + CC (BECN1 Human)
   

   Use

   Tags

   Hexa-Histadine

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF711-pET28a-Hs-delta25-MCAD-NHis wt

   Plasmid

   #85112

   Purpose

   expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta25) MCAD (ACADM Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pF708-pET28a-Hs-delta38-METTL20-NHis wt

   Plasmid

   #85109

   Purpose

   expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta38) METTL20 (METTL20 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-ybbr-HIS-ddFLN4-Fgß

   Plasmid

   #101719

   Purpose

   wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   ddFLN4 fingerprint, Fgß peptide (FGB Human, Dictyostelium discoideum)
   

   Use

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21b-LOV-ipaA L514K L531E

   Plasmid

   #40253

   Depositor

   Brian Kuhlman

   Article

   Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

   Insert

   LOV-ipaA L514K L531E
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L514K; L531E; Jα helix region hybridized (AsLOV2 …

   Promoter

   T7

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

6. FL MALT1 Catalytic Mutant in pET29b-Strep

   Plasmid

   #48969

   Purpose

   Expresses Full length catalytic mutant MALT1 in e.coli

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 isoform A (MALT1 Human)
   

   Use

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Catalytic Mutant C464A

   Promoter

   T7

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pET29-hMIGA2(306-593)-6xhis

   Plasmid

   #192837

   Purpose

   Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Use

   Tags

   6xhis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b-ecDHFR-N23PP/L28F/G51PEKN

   Plasmid

   #118585

   Purpose

   Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N23 mutated to P23, L28 mutated to F28, G51 mutat…

   Promoter

   T7

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a SUMO Nup53xl 162-267

   Plasmid

   #86002

   Purpose

   bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53

   Depositor

   Wolfram Antonin

   Type

   Empty backbone

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid

    #101743

    Purpose

    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4, added 9F in FgßF3

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. delNC C-His Ana GvpC in pET28a

    Plasmid

    #85736

    Purpose

    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (truncated and his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Deletion of N and C terminal regions, addition of…

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-ybbR-HIS-CBM-CohI

    Plasmid

    #58709

    Purpose

    Probing molecule to perform single molecule force spectroscopy experiments

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    CBM-CohI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Alanine 2 to Cysteine in CBM domain

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-NmCas9-Flag D16A+H588A

    Plasmid

    #109033

    Purpose

    D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-Hook3 I136A, I139A, M142A

    Plasmid

    #74617

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28b(+)_S. cerevisiae Hbs1(166-611)

    Plasmid

    #39338

    Depositor

    SeWon Suh

    Insert

    Elongation factor 1 alpha-like protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-ybbR-HIS-10FNIII(x4)-DocI

    Plasmid

    #58712

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    10FNIII(x4)-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. ECMAB - pET22b Ag43 160N 6H Mms6

    Plasmid

    #225539

    Purpose

    Construction of extracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Autotransporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid

    #225139

    Purpose

    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

    Depositor

    Urartu Seker

    Article

    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

    Insert

    Ag43 protein fused to silaffin-R5 and sfGFP
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b Ag43 160N 6H mCherry-R5 AmpR

    Plasmid

    #225141

    Purpose

    Recombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

    Depositor

    Urartu Seker

    Article

    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

    Insert

    Ag43 160N, mCherry
    

    Use

    Tags

    Ag43 protein fused to silaffin-R5 and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b-SUMO-FLAG-TeKaiA-FLAG

    Plasmid

    #229220

    Purpose

    For expression of T. elongatus KaiA protein

    Depositor

    Andy LiWang

    Insert

    kaiA (kaiA T. elongatus)
    

    Use

    Tags

    FLAG and SUMO-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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