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pEF myc-SHP2(Q510H)

Plasmid

#245234

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510H

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q506P)

Plasmid

#245232

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q506P

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510E)

Plasmid

#245233

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510E

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NIC-His6-TEV-SHP2-PTP(WT)

Plasmid

#245216

Purpose

Production of His-tagged SHP2 PTP domain

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 PTP domain

Tags

6xHis

Expression

Bacterial

Mutation

wild-type

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NIC-His6-TEV-SHP2-PTP(Q510E)

Plasmid

#245218

Purpose

Production of His-tagged SHP2 PTP domain

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 PTP domain

Tags

6xHis

Expression

Bacterial

Mutation

Q510E

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(R4A)

Plasmid

#245192

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

R4A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(Q510E)

Plasmid

#245214

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

Q510E

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(Q510K)

Plasmid

#245215

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

Q510K

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(E139A)

Plasmid

#245207

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

E139A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-TurboID

Plasmid

#245241

Purpose

Proximity-labeling proteomics negative control

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

TurboID

Expression

Mammalian

Mutation

wild-type

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1-unspliced-MUT- deltaACAbox

Plasmid

#242753

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

Promoter

CMV

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1-unspliced-MUT- deltaHbox

Plasmid

#242752

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

Promoter

CMV

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1 unspliced-MUT- deltaSNORA13

Plasmid

#242751

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

Promoter

CMV

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NIC-His6-TEV-SHP2-PTP(Q510K)

Plasmid

#245219

Purpose

Production of His-tagged SHP2 PTP domain

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 PTP domain

Tags

6xHis

Expression

Bacterial

Mutation

Q510K

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pTWIST CMV Puro EPB41L4A ORF

Plasmid

#242647

Purpose

Expresses EPB41L4A in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A (EPB41L4A Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-CAG Donor

Plasmid

#241373

Purpose

Bxb1-GT donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-Donor_UbC

Plasmid

#241375

Purpose

Bxb1-GA donor plasmid for cloning of payloads <20 kb with UbC promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBD_PYR1_156

Plasmid

#241285

Purpose

Backbone for PYR1 sensor library assembly from oligopools; domesticated for golden gate cloning

Depositor

Sean Cutler

Article

Tian et al bioRxiv [Preprint]. 2025 May 15:2025.05.

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_2xFlag-dSERT-HDR-donor-HDR

Plasmid

#221835

Purpose

Donor plasmid for inserting 2xFLAG at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

2xFLAG with dSERT homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-1E01

Plasmid

#239013

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of GBGT1

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Leucine 6 to phenylalanine, changed Leuci…

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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