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pMSR:MSMEG_0835:Dendra

Plasmid

#174923

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0835

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_5070:Dendra

Plasmid

#167375

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_5070

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_5487:Dendra

Plasmid

#167440

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_5487

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_5447:Dendra

Plasmid

#167431

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_5447

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2sh-HA

Plasmid

#97072

Purpose

Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

HA

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0310:Dendra

Plasmid

#159051

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0310

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAd-Track HA PGC-1 alpha

Plasmid

#14427

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
FL-BCL2L1

Plasmid

#78860

Purpose

DULIP positive control (prey) for assay establishment

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

BCL2 like 1 (BCL2L1 Human)

Use

Expression vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec22b-shR

Plasmid

#208358

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations introduced to render shRNA …

Promoter

CMV

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEGPN-ACE2

Plasmid

#187483

Purpose

Expression vector - ACE2

Depositor

Lewis Cantley

Article

Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Promoter

EFS

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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Rat SERCA2a (pMT2)

Plasmid

#75183

Purpose

Mammalian expression of ATP2A2

Depositor

Jonathan Lytton, Gary Shull

Article

Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

Insert

ATP2A2

Expression

Mammalian

Promoter

adenovirus major late promoter

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag

Plasmid

#185682

Purpose

Low-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Human, Synthetic)

Tags

HaloTag

Expression

Mammalian

Mutation

K599A

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pNK3203

Plasmid

#219749

Purpose

DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - mcitHispS - tSV40

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2sh-FLAG

Plasmid

#97071

Purpose

Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-mKate-MKLP1

Plasmid

#140570

Purpose

Expresses mKate tagged MKLP1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

MKLP1 (KIF23 Human)

Tags

mKate

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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3019_pETcon-SARS-CoV-2-RBD_E484K

Plasmid

#184404

Purpose

yeast surface display of the SARS-CoV-2 Eta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

E484K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0030:Dendra

Plasmid

#159040

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0030

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag

Plasmid

#185684

Purpose

Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Human, Synthetic)

Tags

HaloTag

Expression

Mammalian

Mutation

K121Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCSGFPnERT(#205)

Plasmid

#184065

Purpose

cyclofen-inducible GFP nuclear relocation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

Insert

eGFP-nls-ERT2

Use

Synthetic Biology

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry-MKLP1

Plasmid

#140562

Purpose

Expresses mCherry tagged MKLP1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

MKLP1 (KIF23 Human)

Tags

2xmCherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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