We narrowed to 8,064 results for: reporter

1. pDNR-SpCas9-Gem

   Plasmid

   #80424

   Purpose

   Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Gem
   

   Use

   CRISPR

   Tags

   Geminin

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CaTCH BC1

   Plasmid

   #154195

   Purpose

   Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   CaTCH BC1
   

   Use

   Lentiviral; Catch barcode labeling

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CaTCH_empty-backbone

   Plasmid

   #157746

   Purpose

   Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Type

   Empty backbone

   Use

   Lentiviral; Catch barcode cloning

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-RYK-V5/HIS

   Plasmid

   #205610

   Purpose

   Expression of human RYK receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   RYK receptor tyrosine kinase (RYK Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

   Plasmid

   #184397

   Purpose

   Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Firefly luciferase beta-globin-PTC39
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   PTC in beta-globin at amino acid position 39

   Promoter

   Tet-On 3G

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-SpCas9-2A-GFP-noITR

   Plasmid

   #118415

   Purpose

   Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRE-UBC-hCD46-T2A-GFP

   Plasmid

   #205457

   Purpose

   lentiviral plasmid for expression of human CD46

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD46 (CD46 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

   Plasmid

   #184398

   Purpose

   Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase beta-globin-PTC39
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   PTC in beta-globin at amino acid position 39

   Promoter

   Tet-On 3G

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-TIE-V5/HIS

   Plasmid

   #205612

   Purpose

   Expression of human TIE receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TIE receptor tyrosine kinase (TIE1 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330.Pten.b

    Plasmid

    #66588

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

11. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

    Plasmid

    #67275

    Purpose

    For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

    Insert

    puromycin resistant gene
    

    Use

    Tags

    linked to red flouresent protein gene (katushka)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF1alpha-rtTA-Antares

    Plasmid

    #197587

    Purpose

    Expresses TET-On reverse transactivator labeled with Antares

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    rtTA-P2A-Antares
    

    Use

    Tags

    Antares

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCVL.Active/Repressed TLR (Sce)

    Plasmid

    #45575

    Purpose

    Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    eGFP with I-Sce I TS
    +3 mCherry
    

    Use

    Lentiviral

    Tags

    T2A dislinker and iRFP

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. TetO-FUW-DN-REST

    Plasmid

    #61533

    Purpose

    Expresses DN-REST in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    REST (REST Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P73, Dominant negative REST (contains the Zf DNA-…

    Promoter

    Available since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p.UTA.2.0 miR-27a-3p

    Plasmid

    #82438

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-27a-3p (MIR27A Human)
    

    Use

    Tags

    Expression

    Mutation

    Three copies hsa-miR-27a-3p Complementary region …

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AMPK(T>A)-SPARK

    Plasmid

    #226256

    Purpose

    phospho-null mutant of AMPK-SPARK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK(T>A)-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    substituted Threonine 20 with Alanine

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330.Pten.a

    Plasmid

    #66587

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. ESR1-Tol2

    Plasmid

    #113382

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    ESR1-enhancer (ESR1 Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

19. ATP9A-Tol2

    Plasmid

    #113385

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    ATP9A-enhancer (ATP9A Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. QSOX1-Tol2

    Plasmid

    #113383

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    QSOX1-enhancer (QSOX1 Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only