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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,308 results for: CAN

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Showing: 1261 - 1270 of 14308 results

  1. pAG mKappa Empty

    Plasmid
    #242954
    Purpose
    Expresses mouse kappa light chain constant region. Users can clone in their desired light chain variable region for mammalian expression; to be used with pAG mIgG2a Empty heavy chain (Plasmid 242953)
    Depositor
    Meghan Rego, Addgene Research Program
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    242954-plasmid-map-sequence-id-485249

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  2. pAAV-Ef1a-Flex-Axon-GCaMP7b

    Plasmid
    #135419
    Purpose
    Can be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.
    Depositor
    Rylan Larsen
    Insert
    axon-jGCaMP7b
    Use
    AAV
    Tags
    6xHis and GAP43 palmitoylation domain
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135419-plasmid-map-sequence-id-271053

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  3. pBW001

    Plasmid
    #131529
    Purpose
    Vector containing the Mu-BsaI-KanR transposon that can be excised with HindIII and BglII for transposition reactions
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    Mu-BsaI-KanR transposon
    Use
    Synthetic Biology
    Available Since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    131529-plasmid-map-sequence-id-263309

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  4. pOpen-Endonuclease IV E. coli

    Plasmid
    #165551
    Purpose
    DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Endonuclease IV E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165551-plasmid-map-sequence-id-339868

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  5. pBlue-hFX-HA(NcoI/EcoRI)

    Plasmid
    #15405
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    insert can be released by digestion with REs NcoI…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    15405-plasmid-map-sequence-id-203076

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  6. Human Coxsackievirus A16 strain G10 2A protease

    Plasmid
    #228632
    Purpose
    Bacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Enterovirus Coxsackievirus A16 2A protease (HEVAgp1 Coxsackievirus 16)
    Tags
    His-SUMO
    Expression
    Bacterial
    Available Since
    Feb. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228632-plasmid-map-sequence-id-461401

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  7. EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed

    Plasmid
    #226590
    Purpose
    Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.
    Depositor
    D. James Surmeier
    Insert
    PSAML-141F,Y115F-GlyR
    Use
    AAV
    Tags
    P2A-Fusion Red
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    226590-plasmid-map-sequence-id-450965

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  8. INT_GFPsp

    Plasmid
    #212144
    Purpose
    Preparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
    Expression
    Bacterial
    Promoter
    pJ23119
    Available Since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212144-plasmid-map-sequence-id-420757

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  9. INT_GFPsp_CamRcargo

    Plasmid
    #212143
    Purpose
    Preparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
    Expression
    Bacterial
    Promoter
    pJ23119
    Available Since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212143-plasmid-map-sequence-id-420641

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  10. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Promoter
    TgTUB1 and Toxoplasma U6
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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Showing: 1261 - 1270 of 14308 results