We narrowed to 4,072 results for: golden gate

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p1300-dCas9-HV5

Plasmid

#239479

Purpose

An intermediate vector is used to assemble three gRNAs via Golden Gate cloning.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

UBQ-dCAS9

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-rtTA-2A-tagBFP-bGH_pKG3264

Plasmid

#246372

Purpose

Expression plasmid for rtTA-2A-tagBFP driven from the CAG promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

rtTA

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-rtTA-2A-mRuby2-bGH_pKG2064

Plasmid

#246373

Purpose

Expression plasmid for rtTA-2A-mRuby2 driven from the EFS promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

rtTA

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-iRFP670-SV40_pKG726

Plasmid

#246374

Purpose

Expression plasmid for iRFP670 driven from the EF1a promoter. Co-transfection marker for transfection experiments.

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

iRFP670

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-AAVS1

Plasmid

#247356

Purpose

Expresses SpCas9 and a sgRNA targeting the human AAVS1 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

AAVS1 (AAVS1 Human)

Use

CRISPR

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGMF2-D

Plasmid

#242206

Purpose

AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in dicots.

Depositor

Kate Creasey Krainer

Article

Abate et al bioRxiv 2025.11.09.687425

Insert

Level1 AtU6-26p::sgRNA2 for subcloning of 20 bp target sequence

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

AtU6-26p

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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IQ-compete reporter (integrating)

Plasmid

#247176

Purpose

Plasmid for genomic integration of the IQ-compete reporter (GFP-TCS-Quencher-CL1) into the YPRCΔ15 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

GFP-TCS-Quencher-CL1

Expression

Yeast

Promoter

TEF1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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IQ-compete reporter (episomal)

Plasmid

#247217

Purpose

Single-copy plasmid expressing the IQ-compete reporter (GFP-TCS-Quencher-CL1).

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

GFP-TCS-Quencher-CL1

Expression

Yeast

Promoter

TEF1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Atp5-DHFR-TEV (integrating)

Plasmid

#247219

Purpose

Plasmid for genomic integration of Atp5 (MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

Atp5 MTS-DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Oxa1-DHFR-TEV (integrating)

Plasmid

#247220

Purpose

Plasmid for genomic integration of Oxa1(MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

Oxa1 MTS-DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pEV749

Plasmid

#246450

Purpose

The plasmid comprises an indicible transcriptional unit encoding EYFP

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

EYFP

Use

Synthetic Biology

Promoter

miniYB

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1540

Plasmid

#246061

Purpose

Bacterial expression of 2TMbeta protein under the pJex promoter

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

2TMbeta

Expression

Bacterial

Promoter

pJEX promoter

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pKG1179_pPV1-EFS

Plasmid

#239690

Purpose

BsaI Golden Gate assembly plasmid encoding the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

EFS promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p0AmpCD-TXdact

Plasmid

#237273

Purpose

Carries the X. dendrorhous act terminator

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Insert

TXdact

Use

Synthetic Biology

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p0AmpCD-TXdgpd

Plasmid

#237272

Purpose

Carries the X. dendrorhous gdh terminator

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Insert

TXdgpd

Use

Synthetic Biology

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p0AmpAB-PXdgdh

Plasmid

#237269

Purpose

Carries the X. dendrorhous gdh promoter

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Insert

PXdgdh

Use

Synthetic Biology

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p0AmpAB-PXdadh4

Plasmid

#237268

Purpose

Carries the X. dendrorhous adh4 promoter

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Insert

PXdadh4

Use

Synthetic Biology

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p0AmpAB-PXdact

Plasmid

#237267

Purpose

Carries the X. dendrorhous act promoter

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Insert

PXdact

Use

Synthetic Biology

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLP-PrhaBAD-pcepA-himar-chlorR

Plasmid

#238492

Purpose

Landing Pad with RhaRS/PrhaBAD, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

RhaRS/PrhaBAD

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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p0AmpAB-PXdUspA

Plasmid

#237241

Purpose

Carries the X. dendrorhous UspA promoter

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Insert

PXdUspA

Use

Synthetic Biology

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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