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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 22,481 results for: SCR

Showing: 5081 - 5120 of 22481 results

  4. pCAROTENE5X

    Plasmid
    #208816
    Purpose
    Encodes bacterial crtEBIY operon under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cell
    Depositor
    Marc Erhardt
    Article
    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:
    Insert
    Five copies of JUB1 binding site within the synthetic promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    JUB1 TF-responsive promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208816-plasmid-map-sequence-id-420948

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  6. pTF-ahpC-rfp

    Plasmid
    #153035
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153035-plasmid-map-sequence-id-318838

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  8. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

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  10. pTF-lacZ-rfp

    Plasmid
    #153037
    Purpose
    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153037-plasmid-map-sequence-id-318855

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  11. pEG BacMam

    Plasmid
    #160451
    Purpose
    vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
    Depositor
    Eric Gouaux
    Article
    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    160451-plasmid-map-sequence-id-319250

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  18. LV-PE_CO-Mini

    Plasmid
    #190338
    Purpose
    Lentiviral vector expressing PE_CO-Mini (Prime editor with codon-optimized and truncated Reverse transcriptase)
    Depositor
    Rasmus Bak
    Article
    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22
    Insert
    PE_CO-Mini, ngRNA and pegRNA for HEK3 CTTins
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    190338-plasmid-map-sequence-id-374563

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  34. pT3TS_Transposase

    Plasmid
    #109768
    Purpose
    Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    Tol2 Transposase
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109768-plasmid-map-sequence-id-225499

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  36. pTF-lacZ

    Plasmid
    #153036
    Purpose
    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153036-plasmid-map-sequence-id-318836

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  37. RIB VP64 R1

    Plasmid
    #161493
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2
    Depositor
    Diethard Mattanovich, Brigitte Gasser
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Targeting R1 in the RIB1 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGAP, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161493-plasmid-map-sequence-id-326164

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  39. Plas-gRNA-Z3EV

    Plasmid
    #157659
    Purpose
    GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.
    Depositor
    Frank Albert
    Article
    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1
    Insert
    GFP-gRNA NatMX Z3EV
    Use
    Insert storage (replicative in e. coli)
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    157659-plasmid-map-sequence-id-309102

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  40. pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid
    #116955
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    116955-plasmid-map-sequence-id-226973

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Showing: 5081 - 5120 of 22481 results