We narrowed to 7,211 results for: CIT

1. pVE13300

   Plasmid

   #137715

   Purpose

   Expresses impLbCas12a in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   impLbCas12a
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET42b-ABE7.10

   Plasmid

   #120398

   Purpose

   Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   Junjiu Huang

   Article

   Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

   Insert

   ABE7.10
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Mammalian codon-optimized

   Promoter

   T7

   Available since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSpCas9(1.1)

   Plasmid

   #138555

   Purpose

   Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_TXK-1/1

   Plasmid

   #91539

   Purpose

   Protein expression and purification of human SH3 domain construct TXK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TXK-1/1 (TXK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNCS-CyOFP

   Plasmid

   #74278

   Purpose

   Constitutive expression of CyOFP in bacteria

   Depositor

   Michael Lin

   Article

   Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   CyOFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV asyn WT

   Plasmid

   #36055

   Depositor

   Hilal Lashuel

   Article

   Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

   Insert

   alpha-synuclein WT (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG/ATP11A(E186Q)-HA

   Plasmid

   #209249

   Purpose

   Mammalian expression of ATP11A mutant E186Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP11A (ATP11A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E186Q

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sc++

   Plasmid

   #155011

   Purpose

   Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

   Insert

   Sc++
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T1227K, Loop Substitution from S. anginosus

   Promoter

   EF1ɑ

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. tdTomato-KIF1A

   Plasmid

   #102850

   Purpose

   Overexpresses KIF1A and cell fill TdTomato

   Depositor

   Erik Dent

   Article

   McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

   Insert

   KIF1A (Kif1a Rat)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. BB44_pmc.DonorR5.TS

    Plasmid

    #199223

    Purpose

    Donor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
    

    Use

    Gene targeting donor plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1 gene promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. NgOE FUNgGW

    Plasmid

    #92231

    Purpose

    Lentiviral expression of human Ng

    Depositor

    Weifeng Xu

    Article

    Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

    Insert

    Ng (NRGN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre

    Plasmid

    #203839

    Purpose

    Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dCre
    

    Use

    AAV

    Tags

    mScarlet-KASH-2A

    Expression

    Mutation

    Enzymatically inactive Cre

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV hSyn FLEx-FRT hM3Dq-mCherry

    Plasmid

    #161575

    Purpose

    Expression of hM3Dq-mCherry in a Flp-dependent fashion

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    hSyn

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS416 Gal Q331K YFP

    Plasmid

    #27450

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3.1-Flag-H2A K118-119R

    Plasmid

    #63564

    Purpose

    Expression of human H2A with K118R and K119R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine 118 and Lysine 119 are now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pOPINK-OTUD2 (full-length, aa 1-348)

    Plasmid

    #61409

    Purpose

    Expresses human OTUD2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD2 (YOD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPINK-OTUD1 (full-length, aa 1-481)

    Plasmid

    #61405

    Purpose

    Expresses human OTUD1 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD1 (OTUD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-shCTRL

    Plasmid

    #181875

    Purpose

    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    control shRNA with no known targets in the mouse genome
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_CaMKIIa_SERT_EYFP

    Plasmid

    #198737

    Purpose

    Serotonin transporter with EYFP fluorescent protein driven by CaMKIIa promoter on a AAV vector

    Depositor

    Lin Tian

    Article

    Vargas et al Science. 2023 Feb 17;379(6633):700-706.

    Insert

    Serotonin transporter and yellow fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

    Plasmid

    #170229

    Purpose

    Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    RHEB (RHEB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Q64L

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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