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pSIN4-DDX6-NT

Plasmid

#240011

Purpose

Expresses human DDX6-NT fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-NT (1-302)

Use

Lentiviral

Tags

3X Flag

Mutation

TTAAAAAAAGATAACATC

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN4-DDX6-ΔIDR

Plasmid

#240012

Purpose

Expresses human DDX6-ΔIDR fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-ΔIDR

Use

Lentiviral

Tags

3X Flag

Mutation

TTAAAAAAAGATAACATC

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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LLPS-pCDH-DDX6-CT

Plasmid

#240014

Purpose

Expresses human DDX6-CT fused to EGFP in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-CT (303-483)

Use

Lentiviral

Tags

6xHis and EGFP

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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LLPS-pCDH-DDX6-ΔDEAD

Plasmid

#240017

Purpose

Expresses human DDX6-ΔDEAD fused to EGFP in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-ΔDEAD

Use

Lentiviral

Tags

6xHis and EGFP

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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LLPS-pCDH-DDX6-ΔIDR

Plasmid

#240016

Purpose

Expresses human DDX6-ΔIDR fused to EGFP in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-ΔIDR

Use

Lentiviral

Tags

6xHis and EGFP

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-Mouse-sgDdx6-1

Plasmid

#240003

Purpose

Expresses Mouse sgDdx6 in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

sgDdx6

Use

Lentiviral

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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LLPS-pCDH-DDX6-NT

Plasmid

#240015

Purpose

Expresses human DDX6-NT fused to EGFP in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-NT (1-302)

Use

Lentiviral

Tags

6xHis and EGFP

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Zf-GFP

Plasmid

#223187

Purpose

Plasmid containing the Zfr/eGFP cassette. Translation of GFP is controlled by splicing modulation of the Zfr by risdiplam.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Type

Empty backbone

Use

AAV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 (N)ZF1-3 (1-173)

Plasmid

#235646

Purpose

Bacterial expression of human CAPN15 (N)ZF1-3 (1-173)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 (N)ZF1-3 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 1-173 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 NZF2 (34-72)

Plasmid

#235645

Purpose

Bacterial expression of human CAPN15 NZF2 (34-72)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 NZF2 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 34-72 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 NZF1 (1-42)

Plasmid

#235644

Purpose

Bacterial expression of human CAPN15 NZF1 (1-42)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 NZF1 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 1-42 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shScg2-CWB

Plasmid

#223659

Purpose

Expresses hM3D(Gq) and a shRNA targetting Scg2 in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shScg2

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-K.l.LEU2

Plasmid

#229229

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-TRP1

Plasmid

#229228

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro human RLIM N581K

Plasmid

#232242

Purpose

Mammalian expression of Human RLIM N581K

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

Human RLIM N581K (RLIM Human)

Expression

Mammalian

Mutation

N581K

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 human RLIM N581K

Plasmid

#232241

Purpose

Protein expression of GST-human RLIM N581K

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

GST-human RLIM N581K (RLIM Human)

Tags

GST

Expression

Bacterial

Mutation

N581K

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP02

Plasmid

#196740

Purpose

Contains PEP2 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PLANT ELICITOR PEPTIDE 2 (PROPEP2 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP03

Plasmid

#196741

Purpose

Contains PEP3 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PLANT ELICITOR PEPTIDE 3 (PROPEP3 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1559

Plasmid

#218590

Purpose

Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimer

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

GES

Tags

ePTS1

Expression

Yeast

Mutation

Erg20(F96W, N127W), Erg20-Erg20 synthetic homodim…

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-D6Y_AroPERFECT

Plasmid

#215634

Purpose

For overexpression of Gal4-DBD D6Y AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

D6Y AroPERFECT

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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