We narrowed to 5,091 results for: codon optimized

1. pSCL.103

   Plasmid

   #184988

   Purpose

   Express Cas9-P2A-Eco1RT

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Cas9-P2A-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL.027

   Plasmid

   #184964

   Purpose

   Express -Eco1 RT and ncRNA in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(wt), a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal7

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCL.102

   Plasmid

   #184987

   Purpose

   Express -Eco1 RT-P2A-Cas9

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-P2A-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCL.006

   Plasmid

   #184970

   Purpose

   Express -Eco1 RT in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Eco1 RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a(+)-HisTim12_CtoA

   Plasmid

   #170281

   Purpose

   Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

   Insert

   yeast Tim12 (TIM12 Budding Yeast)
   

   Use

   Tags

   TEV-cleavable N-terminal His tag (on backbone)

   Expression

   Bacterial

   Mutation

   mutated two non-conserved Cys to Ala

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSN071

   Plasmid

   #166729

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-Mxi1-NLS
   

   Use

   CRISPR

   Tags

   Mxi1 and SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSN072

   Plasmid

   #166730

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-NLS-Mxi1-NLS
   

   Use

   CRISPR

   Tags

   Mxi1 and SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-FNLSHF1-P2A-Puro

   Plasmid

   #136901

   Purpose

   Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHF1
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   R661A,Q695A,Q926A

   Promoter

   EF1s

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSB4C5_J23101-vdh

   Plasmid

   #128130

   Purpose

   Expressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

   Depositor

   Jean-Loup Faulon

   Article

   Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

   Insert

   vdh
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p1147-pCSDest-AcrIIC3Nme-mCherry-miR122BS

    Plasmid

    #126203

    Purpose

    Mammalian expression of AcrIIC3Nme fused to mCherry with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    codon-optimized AcrIIC3Nme
    

    Use

    Tags

    FLAG/NLS and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p1149-pCSDest-AcrIIA4-mCherry-miR122BS

    Plasmid

    #126205

    Purpose

    Mammalian expression of AcrIIA4 fused to mCherry with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    codon-optimized AcrIIA4
    

    Use

    Tags

    FLAG/NLS and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEAST_J23101-HipO

    Plasmid

    #128133

    Purpose

    The cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    HipO
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEAST_J23101-vdh

    Plasmid

    #128134

    Purpose

    The cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    vdh
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pProEx-Htb-cytATL2-6Gly-sfGFP

    Plasmid

    #124065

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-sfGFP
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEP4-SERTR596Q-myc

    Plasmid

    #107483

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R596Q conferring gain of function phenotype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y289I

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCEP4-SERTK605N-myc

    Plasmid

    #107475

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K605N approxmately wildtype activity, decreased surface expression

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K605N

    Promoter

    CMV

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEP4-SERTF465L-myc

    Plasmid

    #107469

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F465L conferring gain of function phenotype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F465L

    Promoter

    CMV

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP4-SERTR462E-myc

    Plasmid

    #107489

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R462E with approximately wildtype activity, decreased surface expression

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R462E

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCEP4-SERTG582K-myc

    Plasmid

    #107497

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G582K conferring gain of function phenotype, decreased surface expression

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G582K

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCEP4-SERTI576K-myc

    Plasmid

    #107496

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576K with approximately wildtype activity, decreased surface expression

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I576K

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge