We narrowed to 5,844 results for: pCas

1. pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA

   Plasmid

   #211819

   Purpose

   lentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishment

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   SpCas9, tracrRNA and blasticidin resistance gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U1a promoter and miniU6 promoter

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

   Plasmid

   #189634

   Purpose

   Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

   Plasmid

   #189632

   Purpose

   Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry

   Plasmid

   #198069

   Purpose

   Mammalian expression of iRFP713(C15S/V256C) and EGFP

   Depositor

   Michiyuki Matsuda

   Insert

   iRFP713(C15S/V256C)
   

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   C15S/V256C mutation

   Promoter

   CAG

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA528 SNX15 MIT residues 263-342

   Plasmid

   #180625

   Purpose

   Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SNX15 (SNX15 Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 263-342

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA528 SPASTIN , F124D, residues 108-200

   Plasmid

   #180629

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200, F124D

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 SPASTIN , L177D, residues 108-200

   Plasmid

   #180634

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200, L177D

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 CHMP4B, residues 156-224, no Cys

   Plasmid

   #180641

   Purpose

   Bacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP4B (CHMP4B Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 156-224

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA528 CHMP4C, residues 156-233, no Cys

   Plasmid

   #180642

   Purpose

   Bacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP4C (CHMP4C Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   residues 316-366

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA528 IST1, residues 315-360, no Cys

    Plasmid

    #180643

    Purpose

    Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    residues 315-360

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCA528 RPS6KC1 MIT, residues 235-316

    Plasmid

    #180622

    Purpose

    Bacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    RPS6KC1 (RPS6KC1 Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 235-316

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

    Plasmid

    #196883

    Purpose

    Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-ZsGreen-bGH
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

    Plasmid

    #199450

    Purpose

    CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    HDR donor template
    

    Use

    CRISPR

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

    Plasmid

    #199453

    Purpose

    CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    HDR donor template
    

    Use

    CRISPR

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA31-pCS2-CMV-stdPCP-p65-HSF1

    Plasmid

    #199462

    Purpose

    Expression of stdPCP-PH in zebrafish embryos

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    stdPCP-PH
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCA30-pCS2-CMV-stdMCP-p65-HSF1

    Plasmid

    #199461

    Purpose

    Expression of stdMCP-PH in zebrafish embryos

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    stdMCP-PH
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

    Plasmid

    #199452

    Purpose

    CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    HDR donor template
    

    Use

    CRISPR

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCA29-pCS2-CMV-Zebrafish NarTag (eGFP)

    Plasmid

    #199460

    Purpose

    Expression of Zebrafish NarTag (eGFP harbors 9XMS2 in its intron) in zebrafish embryos

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    Zebrafish NarTag
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D

    Plasmid

    #198765

    Purpose

    expression of hOCT4 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

    Insert

    hOCT4 236D (POU5F1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S236D

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

    Plasmid

    #195502

    Purpose

    Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA (SOX17 Human)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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