We narrowed to 10,578 results for: poly

1. pcDNA3-PA-B/BRISBANE/60/2008

   Plasmid

   #187920

   Purpose

   Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase acidic protein
   

   Use

   Tags

   UNTAGGED

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-PB1-B/BRISBANE/60/2008

   Plasmid

   #187919

   Purpose

   Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 1
   

   Use

   Tags

   UNTAGGED

   Expression

   Mutation

   Promoter

   CMV Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-PB2-B/BRISBANE/60/2008

   Plasmid

   #187917

   Purpose

   Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 2
   

   Use

   Tags

   UNTAGGED

   Expression

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-GGG-ELP(120nm)-Cys

   Plasmid

   #91572

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-ELP(120nm)-Cys
   

   Use

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-Cys-ELP(120nm)-LPETGG

   Plasmid

   #90472

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   Cys-ELP(120nm)-LPETGG
   

   Use

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

   Plasmid

   #187922

   Purpose

   Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase acidic protein
   

   Use

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUDE1084

   Plasmid

   #168500

   Purpose

   expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

   Depositor

   Jean-Marc Daran

   Article

   Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

   Inserts

   Fncas12a
   T7RNA polymerase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TDH3 and TEF1

   Available since

   Dec. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-miniSOG

   Plasmid

   #193913

   Purpose

   Control plasmid that expresses non-targeted miniSOG in mammalian cells

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CAG-miniSOG-T2A-dTomato

   Plasmid

   #193796

   Purpose

   Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MCV-Rep-

    Plasmid

    #32058

    Depositor

    Patrick Moore

    Article

    Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

    Insert

    MCV-Rep- genome
    

    Use

    Tags

    Expression

    Mutation

    Single cytosine to adenine (C/A) mutation at 2,07…

    Promoter

    none from vector. Insert contains native promoter…

    Available since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. RNAPII_CTD27-52_K5S

    Plasmid

    #98679

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K1838S, K1859S, K1866S, K1873S, K1887S

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWzyE-His

    Plasmid

    #196669

    Purpose

    Expression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Use

    Tags

    TEV cleavable His10

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-fsr1

    Plasmid

    #179866

    Purpose

    Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiae

    Depositor

    Jens Laurids Sørensen

    Article

    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

    Insert

    Fusarubin polyketide synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon optimized for expression in Saccharomyces c…

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CAG-miniSOG-C-Far

    Plasmid

    #193914

    Purpose

    Control plasmid that expresses miniSOG targeted to the cell membrane in mammalian cells

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-2T-RNAPII-A2-14-HA

    Plasmid

    #160689

    Purpose

    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

    Depositor

    Jeffry Corden

    Article

    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

    Insert

    RNA polymerase II CTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains an S-to-A mutation in position 2 of 14 o…

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RNAPII_CTD27-52_N2A

    Plasmid

    #98677

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N1775A, N1782A

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RNAPII_CTD27-52_del1961-1970

    Plasmid

    #98680

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with del1961-1970

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    del1961-1970

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RNAPII_CTD27-52_N2A_S1966C

    Plasmid

    #98682

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966C

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N1775A, N1782A, S1966C

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RNAPII_CTD27-52_K5S_S1966C

    Plasmid

    #98683

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966C

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K1838S, K1859S, K1866S, K1873S, K1887S, S1966C

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-Flag-Polß(L301R/V303R)-IRES-neo

    Plasmid

    #128664

    Purpose

    mammalian expression of Flag-Polß(L301R/V303R) protein with neomycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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