Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 3,244 results for: GAD

Showing: 361 - 390 of 3244 results

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pLPC-Flag-BirA-Linker-Myc-mRap1-I312R

Plasmid

#184645

Purpose

Retroviral expression of BirA Rap1 I312R

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

BirA-rap1-I312R

Use

Retroviral

Tags

BirA FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Hy_pMT Flag-URH49 MUT

Plasmid

#110126

Purpose

Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

URH49 (DDX39A Human)

Tags

Flag

Expression

Insect

Mutation

4 amino acid mutations (RSFS motif changed to KSL…

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG

Plasmid

#127335

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG

Plasmid

#127309

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127317

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/HP + GUG-nLuc-3XFLAG

Plasmid

#127323

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with hairpin in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/AAA-nLuc-3XFLAG-CL1/PEST

Plasmid

#127320

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AAA start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/CUG-nLuc-3XFLAG-PEST

Plasmid

#127313

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/AUU-nLuc-3XFLAG-CL1/PEST

Plasmid

#127319

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUU start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG

Plasmid

#127311

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control GGG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/AAA-stop-nLuc-3XFLAG

Plasmid

#127310

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control AAA start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/BAG1 5′ UTR AUG-nLuc-3xFLAG

Plasmid

#127329

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with BAG1 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/BAG1 5′ UTR CUG-nLuc-3xFLAG

Plasmid

#127328

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon from mRNA with BAG1 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CA-Stat3-t2A-mCherry

Plasmid

#127540

Purpose

Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Stat3 (Stat3 Mouse)

Expression

Mammalian

Mutation

A662C and N664C. Residues changed to Cys renders …

Promoter

CMV

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

Plasmid

#127332

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127321

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

Plasmid

#127314

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127315

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

Plasmid

#127312

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

Plasmid

#127322

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Hy_pMT Flag-Hel25E WT

Plasmid

#110123

Purpose

Expresses Flag-tagged D. melanogaster Hel25E

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

Plasmid

#127308

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127316

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127318

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Hy_pMT Flag-UAP56 MUT

Plasmid

#110124

Purpose

Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

UAP56 (DDX39B Human)

Tags

Flag

Expression

Insect

Mutation

4 mutations (KSLN motif changed to RSFS)

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC-Flag-BirA-Linker-Myc-mRap1-I312R

Plasmid

#184645

Purpose

Retroviral expression of BirA Rap1 I312R

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

BirA-rap1-I312R

Use

Retroviral

Tags

BirA FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Hy_pMT Flag-URH49 MUT

Plasmid

#110126

Purpose

Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

URH49 (DDX39A Human)

Tags

Flag

Expression

Insect

Mutation

4 amino acid mutations (RSFS motif changed to KSL…

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG

Plasmid

#127335

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG

Plasmid

#127309

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127317

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 361 - 390 of 3244 results

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