We narrowed to 757 results for: PA-GFP

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pAS-15 (mm15-21)

Plasmid

#40850

Purpose

Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

let-7–mm15-21

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pAS-12 (mm1-8)

Plasmid

#40847

Purpose

Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

let-7–mm1-8

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
LeGO-iG2-wPRE-pA

Plasmid

#60489

Purpose

eGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)

Depositor

Boris Fehse

Article

Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103

Insert

IRES-eGFP; wPRE; BGH-pA

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

Plasmid

#82704

Purpose

AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

Depositor

Jonathan Ploski

Article

de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

Insert

H1/TO-L Empty gRNA Expression Cassette

Use

AAV and CRISPR

Tags

CMV promoter driving expression of TetR-P2A-GFP-K…

Expression

Mammalian

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

Plasmid

#82703

Purpose

AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

Depositor

Jonathan Ploski

Article

de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

Insert

H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA

Use

AAV and CRISPR

Tags

CMV promoter driving expression of TetR-P2A-GFP-K…

Expression

Mammalian

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

Plasmid

#82704

Purpose

AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

Depositor

Jonathan Ploski

Article

de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

Insert

H1/TO-L Empty gRNA Expression Cassette

Use

AAV and CRISPR

Tags

CMV promoter driving expression of TetR-P2A-GFP-K…

Expression

Mammalian

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

Plasmid

#82703

Purpose

AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

Depositor

Jonathan Ploski

Article

de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

Insert

H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA

Use

AAV and CRISPR

Tags

CMV promoter driving expression of TetR-P2A-GFP-K…

Expression

Mammalian

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

Plasmid

#233371

Purpose

EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Inserts

NES-Kinprola_PKA-mEGFP
FLAG-SV40NLS-Cas9-NLS-T2A-BSD

Use

Lentiviral

Tags

FLAG, NES, and mEGFP

Promoter

EF1α

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

Plasmid

#233373

Purpose

EF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Inserts

NES-Kinprola_on-mEGFP
FLAG-SV40NLS-Cas9-NLS-T2A-BSD

Use

Lentiviral

Tags

FLAG, NES, and mEGFP

Promoter

EF1α

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

Plasmid

#233372

Purpose

EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Inserts

NES-Kinprola_PKA_T/A-mEGFP
FLAG-SV40NLS-Cas9-NLS-T2A-BSD

Use

Lentiviral

Tags

FLAG, NES, and mEGFP

Promoter

EF1α

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-d2eGFP-21

Plasmid

#21972

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Insert

CMV d2eGFP miR-21 sponge

Expression

Mammalian

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only
CMV-d2eGFP-16

Plasmid

#22442

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Insert

CMV d2eGFP miR-16 sponge

Expression

Mammalian

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only
CMV-d2eGFP-20

Plasmid

#21970

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Insert

CMV d2eGFP miR-20 sponge

Expression

Mammalian

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only
pSFG-GFP

Plasmid

#22199

Depositor

Ananda Roy

Article

Hakre et al Mol Cell. 2006 Oct 20. 24(2):301-8.

Insert

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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CMV-d2eGFP-cxcr4

Plasmid

#21967

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only
CMV-d2eGFP-20pf

Plasmid

#21971

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Insert

CMV d2eGFP miR-20 perfect

Expression

Mammalian

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only
CMV-d2eGFP-16pf

Plasmid

#21969

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Insert

CMV d2eGFP miR-16 perfect

Expression

Mammalian

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only
CMV-d2eGFP-empty

Plasmid

#26164

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Insert

Expression

Mammalian

Mutation

…ccgcCTCGAG[sponge sites]GGGCCCgttt… became …ccgc…

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pLSLGFP

Plasmid

#51494

Purpose

pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

GFP
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS and flag

Promoter

virion-sense LIR and 2x35S

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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MXS_mEGFP

Plasmid

#62403

Purpose

MXS_chaining vector with mEGFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mEGFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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