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Plasmid#87830PurposeExpresses lambda-red system with hyg selection markerDepositorInserthyg
UseTagsNoneExpressionBacterialMutationNonePromoterblaAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCherry
Plasmid#155222PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-IVT
Plasmid#205145PurposeIVT template of AcGFP with MCS in frame and polyADepositorInsertAcGFP1
UseIn-vitro transcriptionTagsExpressionMutationPromoterT7Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H21
Plasmid#170335PurposeCFP(ST)_EPAC full length_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(ST)_EPAC full length_Y(UST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H30
Plasmid#170337PurposeCFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP
Plasmid#21181DepositorInsertenhanced green flourescent protein
UseDrosophila expressionTagsExpressionMutationPromoterAvailable sinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H27
Plasmid#170336PurposeCFP(ST)_EPACdDEP _Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(ST)_EPACdDEP _Y(UST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domainPromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-MALAT1M
Plasmid#120382PurposeReporter for RNA nuclear localizationDepositorInsertMALAT1 (MALAT1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H74
Plasmid#170338PurposemTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N3-EPAC1
Plasmid#113110PurposeHuman EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).DepositorInsertRapGEF3 (RAPGEF3 Human)
UseTagsEYFPExpressionMammalianMutationPromoterPCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-haloTyrRS C6
Plasmid#141174PurposeExpression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acidsDepositorInsertC6 HaloTyr tRNA synthetase
UseLentiviralTagsExpressionMammalianMutationL270S, Y271L, N311G, C313TPromoterCMVAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-h_bPAC_cmyc
Plasmid#28134Purposeoptogenetic tool for light modulation of cellular cAMPDepositorInserthumanized photoactivated adenylyl cyclase
UseIn vitro transcriptionTagsMycExpressionMutationPromoterAvailable sinceJune 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
Epac-SH225
Plasmid#191449Purposeexpression of cAMP sensor in yeastDepositorInsertmT2Del-EPACdDEPCD-tdTomato
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.PAC
Plasmid#57825PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter drivenDepositorInsertssgRNA
EFS
PAC
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc-C Med12His
Plasmid#49240Purposeexpresses human Med12 with His tag in insect cellsDepositorInsertMed12 (MED12 Human)
UseTagsHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H188
Plasmid#170349PurposemT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPACE-NG (pRZ5150)
Plasmid#140244PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE_NG
UseTagsExpressionMammalianMutationV82G in TadA*, NG mutations in SpCas9, D10A in Sp…PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pActP-dtrpA1
Plasmid#64713Purposeinducing calcium influx in cultured Drosophila S2 cellsDepositorInsertdtrpA1 (TrpA1 )
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP WT
Plasmid#197569PurposeExpression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
Pyl-tRNA (4 copies)
UseTagsV5-His6ExpressionMammalianMutationPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only