We narrowed to 14,176 results for: TIM

1. pRS413-MET25pr-NFAPoptim

   Plasmid

   #221104

   Purpose

   FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS413-MET25pr-CFAPoptim

   Plasmid

   #221105

   Purpose

   FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS415-CUP1pr-NFAPoptim

   Plasmid

   #221107

   Purpose

   FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS415-CUP1pr-CFAPoptim

   Plasmid

   #221108

   Purpose

   FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-HGM (optimized)

   Plasmid

   #220988

   Purpose

   Adeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion construct

   Depositor

   Joshua Kritzer

   Article

   Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

   Insert

   HaloTag-GFP-mito
   

   Use

   AAV

   Available Since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPBbsr2-OptoSTIM1(CRY2clust)

   Plasmid

   #184715

   Purpose

   OptoSTIM1(CRY2clust)

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   OptoSTIM1
   

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MultiMate-HITI-2c ACTB

   Plasmid

   #206267

   Purpose

   All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
   

   Use

   CRISPR; Recombinant baculovirus production

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MultiMate-PE3 HEK3

   Plasmid

   #206273

   Purpose

   Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
   

   Use

   CRISPR; Recombinant baculovirus production

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MultiMate-PE2 HEK3

   Plasmid

   #206274

   Purpose

   Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
   

   Use

   CRISPR; Recombinant baculovirus production, cre-a…

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Stim1NTD-ABHD16Acyto-mNG

    Plasmid

    #195158

    Purpose

    mammalian expression of ER localized ABHD16A tagged with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    Stim1NTD (STIM1 Human)
    

    Tags

    ABHD16A cytoplasmic domain and mNeonGreen

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mSTIM1_1-250-CFP

    Plasmid

    #69552

    Purpose

    Expresses a STIM1_1-250 fragment mammalian cells

    Depositor

    Yubin Zhou

    Article

    Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

    Insert

    mSTIM1_1-250
    

    Tags

    Cyan Fluorescent Protein (CFP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3.1 (-) + codon optimized Brn3a

    Plasmid

    #86829

    Purpose

    mammalian expression of the transcription factor Brn3a

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    Brn3a (POU4F1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCROPseq-VEX-optimized-scaffold

    Plasmid

    #203321

    Purpose

    Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS413-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221095

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEX-CMV-SP-STIM2(15-746)

    Plasmid

    #18868

    Depositor

    Tobias Meyer

    Article

    Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

    Insert

    STIM2 (STIM2 Human)
    

    Expression

    Mammalian

    Mutation

    Signal peptide(SP) 1-10 from STIM1 and residues 1…

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. BICstim-Gag-dCAS9-VPR

    Plasmid

    #120922

    Purpose

    encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

    Depositor

    Théophile Ohlmann, Philippe Mangeot, Emiliano Ricci

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GAG (FMLV)-dCAS9-VPR
    

    Tags

    none

    Expression

    Mammalian

    Promoter

    hCMV

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEX-SP-YFP-STIM1(23-685)

    Plasmid

    #18857

    Depositor

    Tobias Meyer

    Article

    Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    YFP is inserted between residues 1-23 (signal pep…

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-OptoSTIM1(Cry2(D387A))

    Plasmid

    #70160

    Purpose

    Light-insensitive mutant of OptoSTIM1

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1(Cry2(D387A)) (STIM1 Human, Mustard Weed)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEX-SP-YFP-STIM1(D76A)

    Plasmid

    #18859

    Depositor

    Tobias Meyer

    Article

    Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    D76A mutation - changed aspartate 76 to alanine. …

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

20. p6610 MSCV-IP N-HAonly TIMP1

    Plasmid

    #35009

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    TIMP1 (TIMP1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only