We narrowed to 15,181 results for: Uty

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pTW211b5

Plasmid

#136927

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9(N)-NpuN

Use

CRISPR

Expression

Bacterial

Mutation

see manuscript

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAG247 His-iFAST

Plasmid

#130808

Purpose

Expresses His-tagged iFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAG244 iFAST

Plasmid

#130809

Purpose

Expresses iFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT-MBI (JBEI-3085)

Plasmid

#87950

Purpose

the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

MevT-MBI

Expression

Bacterial

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAG328 td-iFAST

Plasmid

#130811

Purpose

Expresses td-iFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

td-iFAST

Tags

myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTW170b2

Plasmid

#136929

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gVI

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAG330 td-FAST

Plasmid

#130810

Purpose

Expresses td-FAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

td-FAST

Tags

myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_FliC_IRES_mCherry_PGK_tBFP

Plasmid

#85438

Purpose

Lentiviral vector for Gal4 inducible S. Typhi Flagellin with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

S. typhi FliC

Use

Lentiviral

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only
pTW195b63

Plasmid

#136931

Purpose

Directed evolution of SpCas11

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII(N)-npuN

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGGF012

Plasmid

#48849

Purpose

Provides a sulfadiazine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::SulfadiazineR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition sites in SulfR removed …

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTW194b63

Plasmid

#136930

Purpose

Directed evolution of SpCas10

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

npuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pmMaple2-CAM

Plasmid

#101149

Purpose

Fluorescent protein for superresolution imaging

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

E. coli codon optimized mMaple2

Expression

Bacterial

Promoter

pBAD

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGGF002

Plasmid

#48843

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::BastaR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGF008

Plasmid

#48848

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::BastaR::tNOS

Use

Golden gate compatible cloning vector

Mutation

chi sequence in BastaR removed by silent substitu…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGF004

Plasmid

#48845

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::D-AlanineR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGA002

Plasmid

#48813

Purpose

Provides the AP3 promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

AP3 promoter

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site removed by substit…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pT4/HB

Plasmid

#108352

Purpose

Optimized Sleeping Beauty Transposon Vector with MCS

Depositor

Wolfgang Uckert

Article

Wang et al Nucleic Acids Res. 2017 Jan 9;45(1):311-

Type

Empty backbone

Use

Transposon

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Expression

Bacterial

Promoter

PPSP

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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mPiezo1-IRES-eGFP

Plasmid

#80925

Purpose

expresses mouse Piezo1 with GFP

Depositor

Ardem Patapoutian

Article

Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

Insert

mouse Piezo1 CDS (Piezo1 Mouse)

Expression

Mammalian

Available Since

Nov. 25, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-HP1alpha

Plasmid

#17652

Depositor

Tom Misteli

Article

Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

Insert

HP1 alpha (CBX5 Human)

Tags

GFP and His

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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